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In a flotation gradient centrifugation assay, a small proportion of NS1 in Triton X-100 cell lysate consistently co-fractionated with raft markers. Association of NS1 with lipid rafts was detected for all four dengue serotypes, as well as for Japanese encephalitis virus. Analysis of recombinant NS1 forms showed that glycosylated NS1 dimers stably expressed in HEK-293T cells without an additional C-terminal sequence, or with a heterologous transmembrane domain, failed to associate with lipid rafts. In contrast, glycosylphosphatidylinositol-linked recombinant NS1 exhibited a predilection for lipid rafts. These results indicate an association of a minor subpopulation of NS1 with lipid rafts during dengue virus infection and suggest that modification of NS1, possibly lipidation, is required for raft association.<\/jats:p>","DOI":"10.1099\/vir.0.83620-0","type":"journal-article","created":{"date-parts":[[2008,9,16]],"date-time":"2008-09-16T22:18:23Z","timestamp":1221603503000},"page":"2492-2500","source":"Crossref","is-referenced-by-count":97,"title":["Association of dengue virus NS1 protein with lipid rafts"],"prefix":"10.1099","volume":"89","author":[{"given":"Sansanee","family":"Noisakran","sequence":"first","affiliation":[{"name":"Medical Molecular Biology Unit, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"},{"name":"Medical Biotechnology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok 10400, 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