{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:27:26Z","timestamp":1762100846109},"reference-count":25,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,9,1]]},"abstract":"<jats:p>The establishment of clonal infection of hepatitis C virus (HCV) in a small-animal model is important for the analysis of HCV virology. A previous study developed models of molecularly cloned genotype 1a and 2a HCV infection using human hepatocyte-transplanted chimeric mice. This study developed a new model of molecularly cloned genotype 1b HCV infection. A full-length genotype 1b HCV genome, HCV-KT9, was cloned from a serum sample from a patient with severe acute hepatitis. The chimeric mice were inoculated intrahepatically with <jats:italic>in vitro<\/jats:italic>-transcribed HCV-KT9 RNA. Inoculated mice developed viraemia at 2\u2005weeks post-infection, and this persisted for more than 6\u2005weeks. Passage experiments indicated that the sera of these mice contained infectious HCV. Interestingly, a similar clone, HCV-KT1, in which the poly(U\/UC) tract was 29\u2005nt shorter than in HCV-KT9, showed poorer <jats:italic>in vivo<\/jats:italic> infectivity and replication ability. An <jats:italic>in vitro<\/jats:italic> study showed that no virus was produced in the culture medium from HCV-KT9-transfected cells. In conclusion, this study developed a genetically engineered genotype 1b HCV-infected mouse. This mouse model will be useful for the study of HCV virology, particularly the mechanism underlying the variable resistance of HCV genotypes to interferon therapy.<\/jats:p>","DOI":"10.1099\/vir.0.83658-0","type":"journal-article","created":{"date-parts":[[2008,8,27]],"date-time":"2008-08-27T15:45:58Z","timestamp":1219851958000},"page":"2108-2113","source":"Crossref","is-referenced-by-count":31,"title":["Establishment of an infectious genotype 1b hepatitis C virus clone in human hepatocyte chimeric mice"],"prefix":"10.1099","volume":"89","author":[{"given":"Takashi","family":"Kimura","sequence":"first","affiliation":[{"name":"Liver Research Project Center, Hiroshima University, Hiroshima, Japan"},{"name":"Department of Medicine and Molecular Science, Division of Frontier Medical Science, Programs for Biomedical Research, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, 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