{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T22:55:07Z","timestamp":1790463307178,"version":"4.1.0"},"reference-count":32,"publisher":"Microbiology Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,7,1]]},"abstract":"<jats:p>A mouse-adapted strain of human enterovirus 71 (HEV71) was selected by serial passage of a HEV71 clinical isolate (HEV71-26M) in Chinese hamster ovary (CHO) cells (CHO-26M) and in newborn BALB\/c mice (MP-26M). Despite improved growth in CHO cells, CHO-26M did not show increased virulence in newborn BALB\/c mice compared with HEV71-26M. By contrast, infection of newborn mice with MP-26M resulted in severe disease of high mortality. Skeletal muscle was the primary site of replication in mice for both viruses. However, MP-26M infection induced severe necrotizing myositis, whereas CHO-26M infection caused only mild inflammation. MP-26M was also isolated from whole blood, heart, liver, spleen and brain of infected mice. CHO-26M harboured a single mutation within the open reading frame (ORF), resulting in an amino acid substitution of K<jats:sup>149<\/jats:sup>\u2192I in the VP2 capsid protein; two further ORF mutations that resulted in amino acid substitutions were identified in MP-26M, located within the VP1 capsid protein (G<jats:sup>145<\/jats:sup>\u2192E) and the 2C protein (K<jats:sup>216<\/jats:sup>\u2192R). Infectious cDNA clone-derived mutant virus populations containing the mutations identified in CHO-26M and MP-26M were generated in order to study the molecular basis of CHO cell and mouse adaptation. The VP2 (K<jats:sup>149<\/jats:sup>\u2192I) change was responsible only for improved growth in CHO cells and did not lead to increased virulence in mice. Of the two amino acid substitutions identified in MP-26M, the VP1 (G<jats:sup>145<\/jats:sup>\u2192E) mutation alone was sufficient to increase virulence in mice to the level observed in MP-26M-infected mice.<\/jats:p>","DOI":"10.1099\/vir.0.83676-0","type":"journal-article","created":{"date-parts":[[2008,6,17]],"date-time":"2008-06-17T15:47:30Z","timestamp":1213717650000},"page":"1622-1632","source":"Crossref","is-referenced-by-count":129,"title":["The molecular basis of mouse adaptation by human enterovirus 71"],"prefix":"10.1099","volume":"89","author":[{"given":"Beng Hooi","family":"Chua","sequence":"first","affiliation":[{"name":"Division of Virology, Telethon Institute for Child Health Research, Perth, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patchara","family":"Phuektes","sequence":"additional","affiliation":[{"name":"School of Veterinary and Biomedical Sciences, Murdoch University, Perth, Australia"},{"name":"Division of Virology, Telethon 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