{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T15:08:31Z","timestamp":1767625711201},"reference-count":19,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,4,1]]},"abstract":"<jats:p>The toxic effect of NaCl and KCI on growth of the marine yeast <jats:italic>Debaryomyces, hansenii<\/jats:italic> on glucose or glycerol was studied. Above a threshold value, both salts reduced the specific growth rate, specific glucose and glycerol respiration rates and specific glucose fermentation rate, as well as biomass yields. The exponential inhibition constant, <jats:italic>k<\/jats:italic>, and minimum toxic concentration, <jats:italic>c<\/jats:italic>\n                  <jats:sub>min<\/jats:sub>, were similar for all physiological parameters assayed. The effect of either salt on the specific activity of several glycolytic enzymes showed a similar inhibition pattern, although at much lower salt concentrations compared with the physiological parameters. In agreement with published results on glycerol phosphate dehydrogenase stimulation by salt, we present evidence that a general glycolytic flux deviation could occur naturally during salt stress, due to the intrinsic sensitivity of the glycolytic enzymes to intracellular ion concentrations.<\/jats:p>","DOI":"10.1099\/00221287-143-4-1133","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T04:53:32Z","timestamp":1244177612000},"page":"1133-1139","source":"Crossref","is-referenced-by-count":48,"title":["Metabolic flux response to salt-induced stress in the halotoleirant yeast Debaryomyces hansenii"],"prefix":"10.1099","volume":"143","author":[{"given":"M. Luisa","family":"Neves","sequence":"first","affiliation":[{"name":"Departamento de Biologia da Universidade do Minho, Campus de Gualtar 4709 Braga Codex, Portugal"}]},{"given":"Rui P.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Biologia da Universidade do Minho, Campus de Gualtar 4709 Braga Codex, Portugal"}]},{"given":"C\u00e2ndida M.","family":"Lucas","sequence":"additional","affiliation":[{"name":"Departamento de Biologia da Universidade do Minho, Campus de Gualtar 4709 Braga Codex, Portugal"}]}],"member":"345","reference":[{"key":"mic1133-adler","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/BF00427716","article-title":"Polyhydric alcohol production and intracellular amino acid pool in relation to halotolerance of the yeast Debaryomyces hansenii.","volume":"124","author":"Adler","year":"1980","journal-title":"Arch Microbiol"},{"key":"mic1133-adler1","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1128\/JB.162.1.300-306.1985","article-title":"Glycerol metabolism and osmoregulation in the salt-tolerant yeast Debaryomyces hansenii.","volume":"162","author":"Adler","year":"1985","journal-title":"J Bacteriol"},{"key":"mic1133-barnett","article-title":"Yeasts: Characteristics and Identification, 2nd edn. 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