{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T18:44:16Z","timestamp":1778697856588,"version":"3.51.4"},"reference-count":29,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,4,1]]},"abstract":"<jats:p>Most strains of<jats:italic>Staphylococcus aureus<\/jats:italic>express IgG-binding activity and this binding has been considered to be solely mediated by protein A. However, the existence of a second gene in<jats:italic>S. aureus<\/jats:italic>strain 8325-4 encoding an IgG-binding polypeptide was recently reported. This novel IgG-binding polypeptide was found after panning a shotgun phage display library, made from chromosomal DNA, against immobilized human IgG. The complete gene (<jats:italic>sbi<\/jats:italic>) encoding this novel IgG-binding protein, denoted protein Sbi, has now been cloned and sequenced. Analysis of other<jats:italic>S. aureus<\/jats:italic>strains showed that this gene is not unique for strain 8325-4. The protein consists of 436 amino acids and exhibits an immunoglobulin-binding specificity similar to protein A. Furthermore, it is shown that Sbi is highly expressed in strain Newman 4, which shows that IgG-binding activity in<jats:italic>S. aureus<\/jats:italic>can be mediated by proteins other than protein A.<\/jats:p>","DOI":"10.1099\/00221287-144-4-985","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T04:53:32Z","timestamp":1244177612000},"page":"985-991","source":"Crossref","is-referenced-by-count":133,"title":["A second IgG-binding protein in Staphylococcus aureus"],"prefix":"10.1099","volume":"144","author":[{"given":"Lihong","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, S-750 07 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Jacobsson","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, S-750 07 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jozsef","family":"Vasi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, S-750 07 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Lindberg","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, S-750 07 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Frykberg","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, S-750 07 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"ref-1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb01046.x"},{"key":"ref-2","volume-title":"Bacterial Immuno\u03bclobulin-binding Proteins,","volume":"vols 1 and 2","author":"Boyle","year":"(1990)"},{"key":"ref-3","doi-asserted-by":"publisher","DOI":"10.1021\/bi00512a001"},{"key":"ref-4","volume-title":"Staphylococci and Staphylococcal Infections vols","volume":"1 and 2","author":"Easmon","year":"(1983),"},{"key":"ref-5","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.2.4.387-391.1970"},{"key":"ref-6","doi-asserted-by":"crossref","first-page":"822","DOI":"10.4049\/jimmunol.97.6.822","article-title":"Protein A, Pseudoimmune reaction with human y-\u03bclobulin.","volume":"97","author":"Forsgren","year":"(1966)","journal-title":"Journal of Immunology,"},{"key":"ref-7","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1128\/JB.107.1.245-250.1971","article-title":"Protein A mutants of Staphylococcus aureus","volume":"107","author":"Forsgren","year":"(1971)","journal-title":"Journal of Bacteriology,"},{"key":"ref-9","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.15.4133"},{"key":"ref-10","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.43.100189.002111"},{"key":"ref-11","first-page":"878","article-title":"Cloning of ligand-binding domains of bacterial receptors by phage display.","volume":"18","author":"Jacobsson","year":"(1995)","journal-title":"BioTechniques,"},{"key":"ref-12","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1111\/j.1432-1033.1991.tb16468.x","article-title":"Two different genes encode fibronectin binding proteins in Staphylococcus aureus. 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