{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T01:58:58Z","timestamp":1775786338015,"version":"3.50.1"},"reference-count":31,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,12,1]]},"abstract":"<jats:p>Two bacterial strains, G30<jats:sup>T<\/jats:sup> and A1PC16, isolated respectively from raw milk and raw wastewater, were characterized using a polyphasic approach. Chemotaxonomic characterization supported the inclusion of these strains in the genus <jats:italic>Acinetobacter<\/jats:italic>, with Q-8 and Q-9 as the major respiratory quinones, genomic DNA G+C contents within the range observed for this genus (38\u201347 mol%) and C<jats:sub>16\u200a:\u200a0<\/jats:sub>, C<jats:sub>18\u200a:\u200a1<\/jats:sub>\u03c99<jats:italic>c<\/jats:italic> and C<jats:sub>16\u200a:\u200a1<\/jats:sub>\u03c97<jats:italic>c<\/jats:italic>\/iso-C<jats:sub>15\u200a:\u200a0<\/jats:sub> 2-OH as the predominant fatty acids. The observation of 16S rRNA gene sequence similarity lower than 97\u200a% with other <jats:italic>Acinetobacter<\/jats:italic> species with validly published names led to the hypothesis that these isolates could represent a novel species. This hypothesis was supported by comparative analysis of partial sequences of the genes <jats:italic>rpoB<\/jats:italic> and <jats:italic>gyrB<\/jats:italic>, which showed that strains G30<jats:sup>T<\/jats:sup> and A1PC16 did not cluster with any species with validly published names, forming a distinct lineage. DNA\u2013DNA hybridizations confirmed that the two strains were members of the same species, which could be distinguished from their congeners by several phenotypic characteristics. On the basis of these arguments, it is proposed that strains G30<jats:sup>T<\/jats:sup> and A1PC16 represent a novel species, for which the name <jats:italic>Acinetobacter rudis<\/jats:italic> sp. nov. is proposed. The type strain is strain G30<jats:sup>T<\/jats:sup> (\u200a=\u200aLMG 26107<jats:sup>T<\/jats:sup> \u200a=\u200aCCUG 57889<jats:sup>T<\/jats:sup> \u200a=\u200aDSM 24031<jats:sup>T<\/jats:sup> \u200a=\u200aCECT 7818<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.027045-0","type":"journal-article","created":{"date-parts":[[2011,1,15]],"date-time":"2011-01-15T03:00:41Z","timestamp":1295060441000},"page":"2837-2843","source":"Crossref","is-referenced-by-count":45,"title":["Acinetobacter rudis sp. nov., isolated from raw milk and raw wastewater"],"prefix":"10.1099","volume":"61","author":[{"given":"Ivone","family":"Vaz-Moreira","sequence":"first","affiliation":[{"name":"LEPAE \u2013 Departamento de Engenharia Qu\u00edmica, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto, Portugal"},{"name":"CBQF \u2013 Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, 4200-072 Porto, Portugal"}]},{"given":"Ana","family":"Novo","sequence":"additional","affiliation":[{"name":"CBQF \u2013 Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, 4200-072 Porto, Portugal"}]},{"given":"Elionora","family":"Hantsis-Zacharov","sequence":"additional","affiliation":[{"name":"Department of Biology and Environment, Faculty of Natural Sciences, University of Haifa, Oranim, Tivon 36006, Israel"}]},{"given":"Ana Rita","family":"Lopes","sequence":"additional","affiliation":[{"name":"LEPAE \u2013 Departamento de Engenharia Qu\u00edmica, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto, Portugal"}]},{"given":"Margarita","family":"Gomila","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n-Microbiolog\u00eda, Fundaci\u00f3n Hospital Son Ll\u00e0tzer, 07198 Palma de Mallorca, Illes Balears, Spain"}]},{"given":"Olga C.","family":"Nunes","sequence":"additional","affiliation":[{"name":"LEPAE \u2013 Departamento de Engenharia Qu\u00edmica, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto, Portugal"}]},{"given":"C\u00e9lia M.","family":"Manaia","sequence":"additional","affiliation":[{"name":"CBQF \u2013 Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, 4200-072 Porto, Portugal"}]},{"given":"Malka","family":"Halpern","sequence":"additional","affiliation":[{"name":"Department of Biology and Environment, Faculty of Natural Sciences, University of Haifa, Oranim, Tivon 36006, Israel"}]}],"member":"345","reference":[{"key":"r1","first-page":"187","article-title":"Whole-cell fatty acid profiles \u2013 a tool for species and subspecies classification in the Puccinia recondita complex","volume":"87","author":"Ben-Ze\u2019ev","year":"2005","journal-title":"J Plant Pathol"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02486-0"},{"key":"r3","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1128\/MMBR.45.2.316-354.1981","article-title":"Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications","volume":"45","author":"Collins","year":"1981","journal-title":"Microbiol Rev"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1007\/BF02601853"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-47-2-590"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2006.00268.x"},{"key":"r7","first-page":"247","article-title":"[Isolation and characterization of Acinetobacter calcoaceticus from fresh, frozen and ice-stored fish products]","volume":"3","author":"Gennari","year":"1985","journal-title":"Microbiol Alim Nutr"},{"key":"r8","first-page":"131","article-title":"Isolation and characterization of Acinetobacter calcoaceticus from raw, washed and frozen vegetables","volume":"37","author":"Gennari","year":"1986","journal-title":"Arch Vet Ital"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00866-07"},{"key":"r10","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/B978-1-4832-3211-9.50009-7","article-title":"Evolution of protein molecules","volume-title":"Mammalian Protein Metabolism","volume":"vol. 3","author":"Jukes","year":"1969"},{"key":"r11","first-page":"425","article-title":"Genus II. 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