{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T22:06:46Z","timestamp":1762898806882,"version":"3.37.3"},"reference-count":23,"publisher":"Microbiology Society","issue":"Pt_1","funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["PTDC\/BIA-MIC\/105247\/2008"],"award-info":[{"award-number":["PTDC\/BIA-MIC\/105247\/2008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,1,1]]},"abstract":"Two Gram-positive-staining, rod-shaped, endospore-forming isolates (UG-2T<\/jats:sup> and UG-3), with an optimum growth temperature of around 37 \u00b0C and an optimum pH for growth of about 4, were recovered from an acidic effluent of the uranium mill tailing at Urgeiri\u00e7a in Central Portugal. On the basis of 16S rRNA gene sequence similarity, the strains belonged to the family \n \n Sporolactobacillaceae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> and were closely related to \n \n Pullulanibacillus naganoensis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> ATCC 53909T<\/jats:sup> (97.9\u200a%). Unlike \n \n P. naganoensis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>, strains UG-2T<\/jats:sup> and UG-3 grew in medium containing up to 5000 p.p.m. U(VI) but did not hydrolyse pullulan. Chemotaxonomic data also supported the affiliation of strains UG-2T<\/jats:sup> and UG-3 to the genus \n \n Pullulanibacillus<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>. Physiological and biochemical tests along with fatty acid composition allowed differentiation of strains UG-2T<\/jats:sup> and UG-3 from \n \n P. naganoensis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>. It is suggested that strains UG-2T<\/jats:sup> and UG-3 represent a novel species, for which the name \n Pullulanibacillus<\/jats:italic>\n <\/jats:ext-link> \n uraniitolerans<\/jats:italic> is proposed; the type strain is UG-2T<\/jats:sup> (\u200a=\u200aDSM 19429T<\/jats:sup>\u200a=\u200aLMG 24205T<\/jats:sup>). An emended description of the genus \n \n Pullulanibacillus<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> is also proposed. <\/jats:p>","DOI":"10.1099\/ijs.0.040923-0","type":"journal-article","created":{"date-parts":[[2012,2,25]],"date-time":"2012-02-25T05:47:35Z","timestamp":1330148855000},"page":"158-162","source":"Crossref","is-referenced-by-count":16,"title":["Pullulanibacillus uraniitolerans sp. nov., an acidophilic, U(VI)-resistant species isolated from an acid uranium mill tailing effluent and emended description of the genus Pullulanibacillus"],"prefix":"10.1099","volume":"63","author":[{"given":"S\u00f3nia G.","family":"Pereira","sequence":"first","affiliation":[{"name":"Department of Earth Sciences, University of Coimbra, 3000-272 Coimbra, Portugal"}]},{"given":"Luciana","family":"Albuquerque","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal"}]},{"given":"M. Fernanda","family":"Nobre","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Coimbra, 3001-401 Coimbra, Portugal"}]},{"given":"Igor","family":"Tiago","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal"}]},{"given":"Ant\u00f3nio","family":"Ver\u00edssimo","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Coimbra, 3001-401 Coimbra, Portugal"},{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal"}]},{"given":"Alcides","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Earth Sciences, University of Coimbra, 3000-272 Coimbra, Portugal"}]},{"given":"Milton S.","family":"da Costa","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Coimbra, 3001-401 Coimbra, 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