{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T02:23:27Z","timestamp":1778207007865,"version":"3.51.4"},"reference-count":18,"publisher":"Microbiology Society","issue":"Pt_4","funder":[{"name":"NPC Services"},{"name":"Governor\u2019s Biotechnology Initiative of the Louisiana Board of Regents","award":["BOR#015"],"award-info":[{"award-number":["BOR#015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,4,1]]},"abstract":"<jats:p>Two strictly anaerobic bacterial strains, designated IP3-3<jats:sup>T<\/jats:sup> and SBP-1, were isolated from groundwater contaminated by chlorinated alkanes and alkenes at a Superfund Site located near Baton Rouge, Louisiana (USA). Both strains reductively dehalogenate a variety of polychlorinated aliphatic alkanes, including 1,2-dichloroethane, 1,2-dichloropropane, 1,1,2,2-tetrachloroethane, 1,1,2-trichloroethane and 1,2,3-trichloropropane, when provided with hydrogen as the electron donor. To clarify their taxonomic position, strains IP3-3<jats:sup>T<\/jats:sup> and SBP-1 were characterized using a polyphasic approach. Both IP3-3<jats:sup>T<\/jats:sup> and SBP-1 are mesophilic, non-spore-forming, non-motile and Gram-stain-negative. Cells of both strains are irregular cocci with diameters of 0.4\u20131.1 \u00b5m. Both are resistant to ampicillin and vancomycin. The genomic DNA G+C contents of strains IP3-3<jats:sup>T<\/jats:sup> and SBP-1 are 55.5\u00b10.4 and 56.2\u00b10.2 mol% (HPLC), respectively. Major cellular fatty acids include C<jats:sub>18\u200a:\u200a1<\/jats:sub>\u03c99<jats:italic>c<\/jats:italic>, C<jats:sub>16\u200a:\u200a0<\/jats:sub>, C<jats:sub>14\u200a:\u200a0<\/jats:sub> and C<jats:sub>16\u200a:\u200a1<\/jats:sub>\u03c99<jats:italic>c<\/jats:italic>. 16S rRNA gene sequence based phylogenetic analyses indicated that the strains cluster within the phylum <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"class\" xlink:type=\"simple\">\n                     <jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.550\" xlink:type=\"simple\">\n                        <jats:italic>Chloroflexi<\/jats:italic>\n                     <\/jats:ext-link>\n                  <\/jats:named-content> most closely related to but distinct from the species <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"species\" xlink:type=\"simple\">\n                     <jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.14868\" xlink:type=\"simple\">\n                        <jats:italic>Dehalogenimonas lykanthroporepellens<\/jats:italic>\n                     <\/jats:ext-link>\n                  <\/jats:named-content> (96.2\u200a% pairwise similarity) and <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"species\" xlink:type=\"simple\">\n                     <jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.23847\" xlink:type=\"simple\">\n                        <jats:italic>Dehalococcoides mccartyi<\/jats:italic>\n                     <\/jats:ext-link>\n                  <\/jats:named-content> (90.6\u200a% pairwise similarity). Physiological and chemotaxonomic traits as well as phylogenetic analysis support the conclusion that these strains represent a novel species within the genus <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus\" xlink:type=\"simple\">\n                     <jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.14867\" xlink:type=\"simple\">\n                        <jats:italic>Dehalogenimonas<\/jats:italic>\n                     <\/jats:ext-link>\n                  <\/jats:named-content> for which the name <jats:italic>Dehalogenimonas alkenigignens<\/jats:italic> sp. nov. is proposed. The type strain is IP3-3<jats:sup>T<\/jats:sup> (\u200a=\u200aJCM 17062<jats:sup>T<\/jats:sup>\u200a=\u200aNRRL B-59545<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.045054-0","type":"journal-article","created":{"date-parts":[[2012,8,12]],"date-time":"2012-08-12T02:24:22Z","timestamp":1344738262000},"page":"1492-1498","source":"Crossref","is-referenced-by-count":73,"title":["Dehalogenimonas alkenigignens sp. nov., a chlorinated-alkane-dehalogenating bacterium isolated from groundwater"],"prefix":"10.1099","volume":"63","author":[{"given":"Kimberly S.","family":"Bowman","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA"},{"name":"Department of Civil and Environmental Engineering, Louisiana State University, Baton Rouge, LA 70803, USA"}]},{"given":"M. Fernanda","family":"Nobre","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Coimbra, 3001-401 Coimbra, Portugal"}]},{"given":"Milton S.","family":"da Costa","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Coimbra, 3001-401 Coimbra, Portugal"}]},{"given":"Fred A.","family":"Rainey","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Alaska Anchorage, Anchorage, Alaska 99508, USA"},{"name":"Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA"}]},{"given":"William M.","family":"Moe","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, Louisiana State University, Baton Rouge, LA 70803, USA"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1111\/j.1574-6941.2006.00146.x","article-title":"Bacterial diversity of an acidic 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