{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T08:19:47Z","timestamp":1760170787611,"version":"3.37.3"},"reference-count":24,"publisher":"Microbiology Society","issue":"Pt_6","funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["PTDC\/MAR\/109057\/2008"],"award-info":[{"award-number":["PTDC\/MAR\/109057\/2008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,6,1]]},"abstract":"A bacterial strain, A2-57T<\/jats:sup>, recovered from a water sample collected in a uranium mine was taxonomically studied in detail. This strain was a Gram-reaction-negative, rod-shaped bacterium that grew optimally at 25 \u00b0C and at pH 6.0\u20137.0 and had a DNA G+C content of 55.0 mol%. Ubiquinone 8 (UQ-8) was the predominant respiratory quinone and the major fatty acids were C16\u200a:\u200a0<\/jats:sub>, C17\u200a:\u200a0<\/jats:sub> cyclo, summed feature 3 (C16\u200a:\u200a1<\/jats:sub>\u03c96c<\/jats:italic> and\/or \u03c97c<\/jats:italic> and\/or C15\u200a:\u200a0<\/jats:sub> iso 2-OH) and C18\u200a:\u200a1<\/jats:sub>\u03c97c<\/jats:italic>. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A2-57T<\/jats:sup> belonged to the family \n \n Oxalobacteraceae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n and formed a distinct branch with \n \n Glaciimonas immobilis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> Cr9-30T<\/jats:sup>. Strain A2-57T<\/jats:sup> shared approximately 97.3\u200a% 16S rRNA sequence similarity with \n \n G. immobilis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> Cr9-30T<\/jats:sup> and also showed high sequence similarity with members of the genera \n \n Herbaspirillum<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> (96.3\u201397.0\u200a%) and \n \n Collimonas<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> (96.2\u201397.0\u200a%). Although phylogenetically closely related to the type strain of \n \n G. immobilis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>, the low level of DNA\u2013DNA hybridization between the two strains (21.6\u200a%) and several physiological and biochemical properties indicated that the novel strain could be clearly distinguished from \n \n G. immobilis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> LMG 25547T<\/jats:sup>. Therefore, it is concluded that strain A2-57T<\/jats:sup> represents a novel species of the genus \n \n Glaciimonas<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>, for which the name Glaciimonas singularis<\/jats:italic> sp. nov. is proposed. The type strain is A2-57T<\/jats:sup> (\u200a=\u200aCIP 110539T<\/jats:sup>\u200a=\u200aLMG 27070T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.046581-0","type":"journal-article","created":{"date-parts":[[2012,11,24]],"date-time":"2012-11-24T04:42:14Z","timestamp":1353732134000},"page":"2344-2350","source":"Crossref","is-referenced-by-count":14,"title":["Glaciimonas singularis sp. nov., isolated from a uranium mine wastewater treatment plant"],"prefix":"10.1099","volume":"63","author":[{"given":"Ana Paula","family":"Chung","sequence":"first","affiliation":[{"name":"IMAR-CMA, University of Coimbra, 3001-401 Coimbra, Portugal"}]},{"given":"Igor","family":"Tiago","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal"},{"name":"Department of Life Sciences, University of Coimbra, 3001-401 Coimbra, Portugal"}]},{"given":"M. Fernanda","family":"Nobre","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Coimbra, 3001-401 Coimbra, Portugal"}]},{"given":"Ant\u00f3nio","family":"Ver\u00edssimo","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal"},{"name":"Department of Life Sciences, University of Coimbra, 3001-401 Coimbra, Portugal"}]},{"given":"Paula V.","family":"Morais","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Coimbra, 3001-401 Coimbra, Portugal"},{"name":"IMAR-CMA, University of Coimbra, 3001-401 Coimbra, Portugal"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1099\/00207713-36-1-86","article-title":"Characterization of Herbaspirillum seropedicae gen. nov., sp. nov., a root-associated nitrogen-fixing 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