{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T19:50:11Z","timestamp":1771012211352,"version":"3.50.1"},"reference-count":40,"publisher":"Microbiology Society","issue":"Pt_7","funder":[{"name":"Federal Public Planning Service \u2013 Science Policy, Belgium"},{"name":"European Community\u2019s Seventh Framework Programme","award":["FP7-228310"],"award-info":[{"award-number":["FP7-228310"]}]},{"name":"Ghent University Co-Funding"},{"DOI":"10.13039\/501100004543","name":"Chinese Scholarship Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,7,1]]},"abstract":"Five acetic acid bacteria isolates, awK9_3, awK9_4 (\u200a=\u200aLMG 27543), awK9_5 (\u200a=\u200aLMG 28092), awK9_6 and awK9_9, obtained during a study of micro-organisms present in traditionally produced kefir, were grouped on the basis of their MALDI-TOF MS profile with LMG 1530 and LMG 1531T<\/jats:sup>, two strains currently classified as members of the genusAcetobacter<\/jats:italic><\/jats:ext-link><\/jats:named-content>. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences as well as on concatenated partial sequences of the housekeeping genesdnaK<\/jats:italic>,groEL<\/jats:italic>andrpoB<\/jats:italic>indicated that these isolates were representatives of a single novel species together with LMG 1530 and LMG 1531T<\/jats:sup>in the genusAcetobacter<\/jats:italic><\/jats:ext-link><\/jats:named-content>, withAcetobacter aceti<\/jats:italic><\/jats:ext-link><\/jats:named-content>,<\/jats:italic>Acetobacter nitrogenifigens<\/jats:italic><\/jats:ext-link><\/jats:named-content>,Acetobacter oeni<\/jats:italic><\/jats:ext-link><\/jats:named-content>andAcetobacter estunensis<\/jats:italic><\/jats:ext-link><\/jats:named-content>as nearest phylogenetic neighbours. Pairwise similarity of 16S rRNA gene sequences between LMG 1531T<\/jats:sup>and the type strains of the above-mentioned species were 99.7\u200a%, 99.1\u200a%, 98.4\u200a% and 98.2\u200a%, respectively. DNA\u2013DNA hybridizations confirmed that status, while amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) data indicated that LMG 1531T<\/jats:sup>, LMG 1530, LMG 27543 and LMG 28092 represent at least two different strains of the novel species. The major fatty acid of LMG 1531T<\/jats:sup>and LMG 27543 was C18\u200a:\u200a1<\/jats:sub>\u03c97c<\/jats:italic>. The major ubiquinone present was Q-9 and the DNA G+C contents of LMG 1531T<\/jats:sup>and LMG 27543 were 58.3 and 56.7 mol%, respectively. The strains were able to grow ond<\/jats:sc>-fructose andd<\/jats:sc>-sorbitol as a single carbon source. They were also able to grow on yeast extract with 30\u200a%d<\/jats:sc>-glucose and on standard medium with pH 3.6 or containing 1\u200a% NaCl. They had a weak ability to produce acid fromd<\/jats:sc>-arabinose. These features enabled their differentiation from their nearest phylogenetic neighbours. The nameAcetobacter sicerae<\/jats:italic>sp. nov. is proposed with LMG 1531T<\/jats:sup>(\u200a=\u200aNCIMB 8941T<\/jats:sup>) as the type strain.<\/jats:p>","DOI":"10.1099\/ijs.0.058354-0","type":"journal-article","created":{"date-parts":[[2014,4,25]],"date-time":"2014-04-25T02:21:31Z","timestamp":1398392491000},"page":"2407-2415","source":"Crossref","is-referenced-by-count":33,"title":["Acetobacter sicerae sp. nov., isolated from cider and kefir, and identification of species of the genus Acetobacter by dnaK, groEL and rpoB sequence analysis"],"prefix":"10.1099","volume":"64","author":[{"given":"Leilei","family":"Li","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium"}]},{"given":"Anneleen","family":"Wieme","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Brewing, Faculty of Applied Bioscience Engineering, University Ghent, Valentin Vaerwyckweg 1, B-9000 Ghent, Belgium"},{"name":"Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium"}]},{"given":"Freek","family":"Spitaels","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium"}]},{"given":"Tom","family":"Balzarini","sequence":"additional","affiliation":[{"name":"Research Group of Industrial Microbiology and Food Biotechnology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium"}]},{"given":"Olga C.","family":"Nunes","sequence":"additional","affiliation":[{"name":"LEPAE \u2013 Departamento de Engenharia Qu\u00edmica, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto, Portugal"}]},{"given":"C\u00e9lia M.","family":"Manaia","sequence":"additional","affiliation":[{"name":"CBQF \u2013 Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, 4200-072 Porto, Portugal"}]},{"given":"Anita","family":"Van Landschoot","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Brewing, Faculty of Applied Bioscience Engineering, University Ghent, Valentin Vaerwyckweg 1, B-9000 Ghent, Belgium"}]},{"given":"Luc","family":"De Vuyst","sequence":"additional","affiliation":[{"name":"Research Group of Industrial Microbiology and Food Biotechnology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium"}]},{"given":"Ilse","family":"Cleenwerck","sequence":"additional","affiliation":[{"name":"BCCM\/LMG Bacteria Collection, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium"}]},{"given":"Peter","family":"Vandamme","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. 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