{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T13:16:09Z","timestamp":1773926169840,"version":"3.50.1"},"reference-count":33,"publisher":"Microbiology Society","issue":"Pt_11","funder":[{"name":"FCT"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,11,1]]},"abstract":"A Gram-positive, rod-shaped, endospore-forming \n \n Bacillus<\/jats:italic> \n <\/jats:ext-link>\n <\/jats:named-content>isolate, Bi.FFUP1<\/jats:sub>\n T<\/jats:sup>, recovered in Portugal from a health product was subjected to a polyphasic study and compared with the type strains of \n \n Bacillus pumilus<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>, \n \n Bacillus safensis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>, \n \n Bacillus altitudinis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> and \n \n Bacillus xiamenensis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>, the phenotypically and genotypically most closely related species. Acid production from cellobiose, d<\/jats:sc>-glucose and d<\/jats:sc>-mannose and absence of acid production from d<\/jats:sc>-arabinose, erythritol, inositol, maltose, mannitol, raffinose, rhamnose, sorbitol, starch and l<\/jats:sc>-tryptophan discriminated this new isolate from the type strains of the most closely related species. Additionally, a significant different protein and carbohydrate signature was evidenced by spectroscopic techniques, matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry and Fourier transform IR spectroscopy with attenuated total reflectance. Using a chemometric approach, the score plot generated by principal component analysis clearly delineated the isolate as a separate cluster. The quinone system for strain Bi.FFUP1<\/jats:sub>\n T<\/jats:sup> comprised predominantly menaquinone MK-7 and major polar lipids were diphosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid. Strain Bi.FFUP1<\/jats:sub>\n T<\/jats:sup> showed \u226599\u200a% 16S rRNA gene sequence similarity to \n \n B. safensis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> FO-036bT<\/jats:sup>, \n \n B. pumilus<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> (7061T<\/jats:sup> and SAFR-032), \n \n B. altitudinis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> 41KF2bT<\/jats:sup> and \n \n B. xiamenensis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> HYC-10T<\/jats:sup>. Differences in strain Bi.FFUP1<\/jats:sub>\n T<\/jats:sup> \n gyrB<\/jats:italic> and rpoB<\/jats:italic> sequences in comparison with the most closely related species and DNA\u2013DNA hybridization experiments with Bi.FFUP1<\/jats:sub>\n T<\/jats:sup> and \n \n B. pumilus<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> ATCC 7061T<\/jats:sup>, \n \n B. safensis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> FO-036bT<\/jats:sup>, \n \n B. altitudinis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> 41KF2bT<\/jats:sup> and \n \n B. xiamenensis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> HYC-10T<\/jats:sup> gave relatedness values of 39.6\u200a% (reciprocal 38.0\u200a%), 49.9\u200a% (reciprocal 42.9\u200a%), 61.9\u200a% (reciprocal 52.2\u200a%) and 61.7\u200a% (reciprocal 49.2\u200a%), respectively, supported the delineation of strain Bi.FFUP1<\/jats:sub>\n T<\/jats:sup> as a representative of a novel species of the genus \n \n Bacillus<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>, for which the name \n \n Bacillus<\/jats:italic> \n <\/jats:ext-link>\n <\/jats:named-content>\n invictae<\/jats:italic> sp. nov. is proposed, with strain Bi.FFUP1<\/jats:sub>\n T<\/jats:sup> (\u200a=\u200aDSM 26896T<\/jats:sup>\u200a=\u200aCCUG 64113T<\/jats:sup>) as the type strain.<\/jats:p>","DOI":"10.1099\/ijs.0.067850-0","type":"journal-article","created":{"date-parts":[[2014,8,30]],"date-time":"2014-08-30T02:08:03Z","timestamp":1409364483000},"page":"3867-3876","source":"Crossref","is-referenced-by-count":19,"title":["Bacillus invictae sp. nov., isolated from a health product"],"prefix":"10.1099","volume":"64","author":[{"given":"Raquel","family":"Branquinho","sequence":"first","affiliation":[{"name":"REQUIMTE, Laborat\u00f3rio de Microbiologia, Departamento de Ci\u00eancias Biol\u00f3gicas, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"}]},{"given":"Clara","family":"Sousa","sequence":"additional","affiliation":[{"name":"REQUIMTE, Laborat\u00f3rio de Microbiologia, Departamento de Ci\u00eancias Biol\u00f3gicas, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"}]},{"given":"Hugo","family":"Os\u00f3rio","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, Universidade do Porto, Porto, Portugal"},{"name":"IPATIMUP, Instituto de Patologia e Imunologia Molecular da Universidade do Porto, Porto, Portugal"}]},{"given":"Lu\u00eds","family":"Meirinhos-Soares","sequence":"additional","affiliation":[{"name":"INFARMED, Lisboa, Portugal"}]},{"given":"Jo\u00e3o","family":"Lopes","sequence":"additional","affiliation":[{"name":"Departamento de Farm\u00e1cia Gal\u00e9nica e Tecnologia Farmacutica, Faculdade de Farm\u00e1cia, Universidade de Lisboa, Lisboa, Portugal"},{"name":"REQUIMTE, Laborat\u00f3rio de Qu\u00edmica Aplicada, Departamento de Ci\u00eancias Qu\u00edmicas, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"}]},{"given":"Jo\u00e3o A.","family":"Carri\u00e7o","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina de Lisboa, Instituto de Microbiologia, Instituto de Medicina Molecular, Lisboa, Portugal"}]},{"given":"Hans-J\u00fcrgen","family":"Busse","sequence":"additional","affiliation":[{"name":"Microbiology and Genetics Institute, University of Wien, Vienna, Austria"}]},{"given":"Amir","family":"Abdulmawjood","sequence":"additional","affiliation":[{"name":"Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Hannover, Germany"}]},{"given":"G\u00fcnter","family":"Klein","sequence":"additional","affiliation":[{"name":"Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Hannover, Germany"}]},{"given":"Peter","family":"K\u00e4mpfer","sequence":"additional","affiliation":[{"name":"Department of Applied Microbiology, Justus Liebig University Giessen, Giessen, Germany"}]},{"given":"Manuela E.","family":"Pintado","sequence":"additional","affiliation":[{"name":"CBQF, Centro de Biotecnologia e Qu\u00edmica Fina, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Porto, Portugal"}]},{"given":"Lu\u00edsa V.","family":"Peixe","sequence":"additional","affiliation":[{"name":"REQUIMTE, Laborat\u00f3rio de Microbiologia, Departamento de Ci\u00eancias Biol\u00f3gicas, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.1099\/ijs.0.65440-0","article-title":"Complete rpoB gene sequencing as a suitable supplement to DNA\u2013DNA hybridization for bacterial species and genus delineation","volume":"58","author":"Ad\u00e9kambi","year":"2008","journal-title":"Int J Syst Evol Microbiol"},{"key":"r2","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.tim.2008.09.008","article-title":"The rpoB gene as a tool 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spores","volume":"80","author":"Lasch","year":"2008","journal-title":"Anal Chem"},{"key":"r19","doi-asserted-by":"crossref","first-page":"e80097","DOI":"10.1371\/journal.pone.0080097","article-title":"Phylogenetic diversity of the Bacillus pumilus group and the marine ecotype revealed by multilocus sequence analysis","volume":"8","author":"Liu","year":"2013","journal-title":"PLoS ONE"},{"key":"r20","first-page":"21","article-title":"Genus Bacillus Cohn 1872","volume-title":"Bergey\u2019s Manual of Systematic Bacteriology","volume":"vol. 3","author":"Logan","year":"2009"},{"key":"r21","volume-title":"A User-Friendly Guide to Multivariate Calibration and Classification","author":"N\u00e6s","year":"2002"},{"key":"r22","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1038\/351081a0","article-title":"Microbiological characterizations by FT-IR spectroscopy","volume":"351","author":"Naumann","year":"1991","journal-title":"Nature"},{"key":"r24","first-page":"406","article-title":"The 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