{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T07:00:45Z","timestamp":1778223645346,"version":"3.51.4"},"reference-count":45,"publisher":"Microbiology Society","issue":"Pt_1","funder":[{"name":"Hercules Foundation"},{"name":"Research Foundation-Flanders"},{"name":"University Research Council"},{"name":"Federal Public Planning Service - Science Policy, Belgium"},{"name":"Ghent University Co-Funding"},{"DOI":"10.13039\/501100004543","name":"Chinese Scholarship Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,1,1]]},"abstract":"In the frame of a bumble bee gut microbiota study, acetic acid bacteria (AAB) were isolated using a combination of direct isolation methods and enrichment procedures. MALDI-TOF MS profiling of the isolates and a comparison of these profiles with profiles of established AAB species identified most isolates asAsaia astilbis<\/jats:italic><\/jats:ext-link><\/jats:named-content>or as \u2018Commensalibacter intestini\u2019<\/jats:italic>, except for two isolates (R-52486 and LMG 28161T<\/jats:sup>) that showed an identical profile. A nearly complete 16S rRNA gene sequence of strain LMG 28161T<\/jats:sup>was determined and showed the highest pairwise similarity toSaccharibacter floricola<\/jats:italic><\/jats:ext-link><\/jats:named-content>S-877T<\/jats:sup>(96.5\u200a%), which corresponded with genus level divergence in the familyAcetobacteraceae<\/jats:italic><\/jats:ext-link><\/jats:named-content>. Isolate LMG 28161T<\/jats:sup>was subjected to whole-genome shotgun sequencing; a 16S\u201323S rRNA internal transcribed spacer (ITS) sequence as well as partial sequences of the housekeeping genesdnaK<\/jats:italic>,groEL<\/jats:italic>andrpoB<\/jats:italic>were extracted for phylogenetic analyses. The obtained data confirmed that this isolate is best classified into a new genus in the familyAcetobacteraceae<\/jats:italic><\/jats:ext-link><\/jats:named-content>. The DNA G+C content of strain LMG 28161T<\/jats:sup>was 54.9 mol%. The fatty acid compositions of isolates R-52486 and LMG 28161T<\/jats:sup>were similar to those of established AAB species [with C18\u200a:\u200a1<\/jats:sub>\u03c97c<\/jats:italic>(43.1\u200a%) as the major component], but the amounts of fatty acids such as C19\u200a:\u200a0<\/jats:sub>cyclo \u03c98c<\/jats:italic>, C14\u200a:\u200a0<\/jats:sub>and C14\u200a:\u200a0<\/jats:sub>2-OH enabled to differentiate them. The major ubiquinone was Q-10. Both isolates could also be differentiated from the known genera of AAB by means of biochemical characteristics, such as their inability to oxidize ethanol to acetic acid, negligible acid production from melibiose, and notable acid production fromd<\/jats:sc>-fructose, sucrose andd<\/jats:sc>-mannitol. In addition, they produced 2-keto-d<\/jats:sc>-gluconate, but not 5-keto-d<\/jats:sc>-gluconate fromd<\/jats:sc>-glucose. Therefore, the nameBombella intestini<\/jats:italic>gen nov., sp. nov. is proposed for this new taxon, with LMG 28161T<\/jats:sup>(\u200a=\u200aDSM 28636T<\/jats:sup>\u200a=\u200aR-52487T<\/jats:sup>) as the type strain of the type species.<\/jats:p>","DOI":"10.1099\/ijs.0.068049-0","type":"journal-article","created":{"date-parts":[[2014,10,22]],"date-time":"2014-10-22T07:29:24Z","timestamp":1413962964000},"page":"267-273","source":"Crossref","is-referenced-by-count":62,"title":["Bombella intestini gen. nov., sp. nov., an acetic acid bacterium isolated from bumble bee crop"],"prefix":"10.1099","volume":"65","author":[{"given":"Leilei","family":"Li","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium"}]},{"given":"Jessy","family":"Praet","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium"}]},{"given":"Wim","family":"Borremans","sequence":"additional","affiliation":[{"name":"Research Group of Industrial Microbiology and Food Biotechnology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium"}]},{"given":"Olga C.","family":"Nunes","sequence":"additional","affiliation":[{"name":"LEPAE \u2013 Departamento de Engenharia Qu\u00edmica, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto, Portugal"}]},{"given":"C\u00e9lia M.","family":"Manaia","sequence":"additional","affiliation":[{"name":"CBQF \u2013 Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, 4200-072 Porto, Portugal"}]},{"given":"Ilse","family":"Cleenwerck","sequence":"additional","affiliation":[{"name":"BCCM\/LMG Bacteria Collection, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium"}]},{"given":"Ivan","family":"Meeus","sequence":"additional","affiliation":[{"name":"Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Belgium"}]},{"given":"Guy","family":"Smagghe","sequence":"additional","affiliation":[{"name":"Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Belgium"}]},{"given":"Luc","family":"De Vuyst","sequence":"additional","affiliation":[{"name":"Research Group of Industrial Microbiology and Food Biotechnology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium"}]},{"given":"Peter","family":"Vandamme","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. 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