{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T06:41:11Z","timestamp":1776321671582,"version":"3.50.1"},"reference-count":33,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,11,1]]},"abstract":"<jats:p>A novel thermophilic, obligately methylotrophic, methanogenic archaeon, strain L2FAW<jats:sup>T<\/jats:sup>, was isolated from a thermophilic laboratory-scale upflow anaerobic sludge blanket reactor fed with methanol as the carbon and energy source. Cells of strain L2FAW<jats:sup>T<\/jats:sup> were non-motile, irregular cocci, 0\u00b77\u20131\u00b75\u2005\u03bcm in diameter and usually occurred singly (sometimes forming clusters of two or four cells). The cells stained Gram-negative and lysed immediately in 0\u00b71\u200a% (w\/v) SDS. Growth was inhibited by chloramphenicol and tetracycline, but not by penicillin, bacitracin, spectinomycin, vancomycin or kanamycin. Methanol and mono-, di- and trimethylamine were used as substrates, but H<jats:sub>2<\/jats:sub>\/CO<jats:sub>2<\/jats:sub>, formate, acetate, propanol, dimethyl sulfide and methanethiol were not. The temperature range for growth was 42\u201358\u200a\u00b0C, with an optimum at 50\u200a\u00b0C. The fastest growth was observed at a salinity below 100\u2005mM NaCl; no growth occurred above 300\u2005mM NaCl. The optimal pH for growth was 6\u00b75; growth was observed from pH\u20055 to pH\u20057\u00b75. The G+C content of the genomic DNA was 37\u00b76\u2005mol%. Analysis of the 16S rRNA gene sequence and the partial methyl-CoM reductase gene sequence revealed that the organism was phylogenetically closely related to <jats:italic>Methanomethylovorans hollandica<\/jats:italic> DMS1<jats:sup>T<\/jats:sup> (98\u200a% similarity for the 16S rRNA gene sequence and 91\u200a% similarity for the methyl-CoM reductase gene sequence). The DNA\u2013DNA relatedness between L2FAW<jats:sup>T<\/jats:sup> and <jats:italic>Methanomethylovorans hollandica<\/jats:italic> DMS1<jats:sup>T<\/jats:sup> was 46\u200a%. On the basis of these results, strain L2FAW<jats:sup>T<\/jats:sup> (=DSM 17232<jats:sup>T<\/jats:sup>=ATCC BAA-1173<jats:sup>T<\/jats:sup>) represents the type strain of a novel species, for which the name <jats:italic>Methanomethylovorans thermophila<\/jats:italic> sp. nov. is proposed.<\/jats:p>","DOI":"10.1099\/ijs.0.63818-0","type":"journal-article","created":{"date-parts":[[2005,11,9]],"date-time":"2005-11-09T21:07:56Z","timestamp":1131570476000},"page":"2465-2470","source":"Crossref","is-referenced-by-count":96,"title":["Methanomethylovorans thermophila sp. nov., a thermophilic, methylotrophic methanogen from an anaerobic reactor fed with methanol"],"prefix":"10.1099","volume":"55","author":[{"given":"B.","family":"Jiang","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands"}]},{"given":"S. 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