{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T08:07:28Z","timestamp":1765354048350,"version":"3.32.0"},"reference-count":19,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,8,1]]},"abstract":"<jats:p>A Gram-positive bacterium, designated B22<jats:sup>T<\/jats:sup>, was isolated from potting soil produced in Portugal. This organism is a catalase-positive, oxidase-negative, motile, spore-forming, aerobic rod that grows optimally at 37\u200a\u00b0C and pH\u20058.0\u20138.5. Optimal growth occurs in media containing 1\u200a% (w\/v) NaCl, although the organism can grow in 0\u20138\u200a% NaCl. The cell wall peptidoglycan is of the A4<jats:italic>\u03b1<\/jats:italic>type with a cross-linkage containing<jats:sc>d<\/jats:sc>-Asp. The major respiratory quinone is menaquinone 7 and the major fatty acids are anteiso-15\u200a:\u200a0, anteiso-17\u200a:\u200a0 and iso-15\u200a:\u200a0. The DNA G+C content is 37.9\u2005mol%. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain B22<jats:sup>T<\/jats:sup>formed a new branch within the family<jats:italic>Bacillaceae<\/jats:italic>. The novel isolate is phylogenetically closely related to members of genera of moderately halophilic bacilli and formed a coherent cluster with species of the genera<jats:italic>Salinibacillus<\/jats:italic>,<jats:italic>Virgibacillus<\/jats:italic>,<jats:italic>Oceanobacillus<\/jats:italic>and<jats:italic>Lentibacillus<\/jats:italic>, supported by bootstrap analysis at a confidence level of 71\u200a%. Strain B22<jats:sup>T<\/jats:sup>exhibited 16S rRNA gene pairwise sequence similarity values of 94.7\u201394.3\u200a% with members of the genus<jats:italic>Salinibacillus<\/jats:italic>, 95.1\u201392.8\u200a% with members of the genus<jats:italic>Virgibacillus<\/jats:italic>, 94.7\u201393.2\u200a% with members of the genus<jats:italic>Oceanobacillus<\/jats:italic>and 93.1\u201392.3\u200a% with members of the genus<jats:italic>Lentibacillus<\/jats:italic>. On the basis of phylogenetic analysis and physiological and biochemical characteristics, it is proposed that strain B22<jats:sup>T<\/jats:sup>represents a novel species in a new genus,<jats:italic>Paucisalibacillus globulus<\/jats:italic>gen. nov., sp. nov. Strain B22<jats:sup>T<\/jats:sup>(=LMG 23148<jats:sup>T<\/jats:sup>=CIP 108857<jats:sup>T<\/jats:sup>) is the type strain of<jats:italic>Paucisalibacillus globulus<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/ijs.0.64261-0","type":"journal-article","created":{"date-parts":[[2006,8,10]],"date-time":"2006-08-10T17:57:37Z","timestamp":1155232657000},"page":"1841-1845","source":"Crossref","is-referenced-by-count":40,"title":["Paucisalibacillus globulus gen. nov., sp. nov., a Gram-positive bacterium isolated from potting soil"],"prefix":"10.1099","volume":"56","author":[{"given":"In\u00eas","family":"Nunes","sequence":"first","affiliation":[{"name":"Departamento de Zoologia and Centro de Neuroci\u00eancias de Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal"}]},{"given":"Igor","family":"Tiago","sequence":"additional","affiliation":[{"name":"Departamento de Zoologia and Centro de Neuroci\u00eancias de Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal"}]},{"given":"Ana Lu\u00edsa","family":"Pires","sequence":"additional","affiliation":[{"name":"Departamento de Zoologia and Centro de Neuroci\u00eancias de Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal"}]},{"given":"Milton S.","family":"da Costa","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica and Centro de Neuroci\u00eancias de Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal"}]},{"given":"Ant\u00f3nio","family":"Ver\u00edssimo","sequence":"additional","affiliation":[{"name":"Departamento de Zoologia and Centro de Neuroci\u00eancias de Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1128\/JCM.14.3.298-303.1981","article-title":"Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens","volume":"14","author":"Edelstein","year":"1981","journal-title":"J Clin Microbiol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1007\/BF01734359","article-title":"Evolutionary trees from DNA sequences: a maximum likelihood approach","volume":"17","author":"Felsenstein","year":"1981","journal-title":"J Mol Evol"},{"key":"R3","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1111\/j.1558-5646.1985.tb00420.x","article-title":"Confidence limits on phylogenies: an approach using the bootstrap","volume":"39","author":"Felsenstein","year":"1985","journal-title":"Evolution"},{"key":"R4","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1099\/00207713-48-1-99","article-title":"Virgibacillus : a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). 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