{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T04:20:24Z","timestamp":1744258824112,"version":"3.40.4"},"reference-count":50,"publisher":"Microbiology Society","issue":"4","funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["UIDB\/00285\/2020, LA\/P\/0112\/2020, UIDB\/50017\/2020 + UIDP\/50017\/2020+LA\/P\/0094\/2020"],"award-info":[{"award-number":["UIDB\/00285\/2020, LA\/P\/0112\/2020, UIDB\/50017\/2020 + UIDP\/50017\/2020+LA\/P\/0094\/2020"]}]},{"name":"Horizon 2020","award":["821096"],"award-info":[{"award-number":["821096"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["PD\/BD\/113605\/2015"],"award-info":[{"award-number":["PD\/BD\/113605\/2015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,4,9]]},"abstract":"<jats:p>Bacterial strain SL12-8<jats:sup>T<\/jats:sup> was characterized and isolated from the skin microbiota of <jats:italic>Pelophylax perezi<\/jats:italic>, the Perez\u2019s frog. Strain SL12-8<jats:sup>T<\/jats:sup> stained Gram-negative and formed rod-shaped cells that grew optimally at 25 \u00b0C and pH 7.0\u20137.5. The G+C content of the DNA was 66.2 mol%. Ubiquinone 8 was the respiratory quinone identified in the studied strain and the most closely related taxon. The major fatty acids were summed in feature 3 and summed in feature 8 and C<jats:sub>16:0<\/jats:sub>, representing 84% of the total fatty acids. Phylogenetic analyses based on the 16S rRNA gene sequence placed strain SL12-8<jats:sup>T<\/jats:sup> within the order <jats:italic>Burkholderiales<\/jats:italic> in a distinct lineage. The 16S rRNA gene sequence similarities of strain SL12-8<jats:sup>T<\/jats:sup> to that of <jats:italic>Rubrivivax albus<\/jats:italic>, <jats:italic>Scleromatobacter humisilvae<\/jats:italic>, <jats:italic>Piscinibacter aquaticus<\/jats:italic>, <jats:italic>Azohydromonas caseinilytica<\/jats:italic> and <jats:italic>Aquincola agrisoli<\/jats:italic> were 94.41, 94.08, 93.72, 93.72 and 93.64%, respectively. The draft genome sequence of strain SL12-8<jats:sup>T<\/jats:sup> comprises 3,115,197 bases with a 313-fold mapped genome coverage. The assembled genome consists of 53 large contigs with more than 500 bp, and the genome encodes 2,814 putative coding sequences. The analysis of the available genomes from the closest genera showed 124 core genes that reveal a novel genus-level clade including the strain SL12-8<jats:sup>T<\/jats:sup>. Analysis of the SL12-8<jats:sup>T<\/jats:sup> genome revealed the presence of the beta-lactone and terpene biosynthetic gene clusters. The phylogenomic, phylogenetic, phenotypic and chemotaxonomic data showed that strain SL12-8<jats:sup>T<\/jats:sup> (=UCCCB 131<jats:sup>T<\/jats:sup>=CECT 30762<jats:sup>T<\/jats:sup>) represents the type of a novel species and genus, for which we propose the name <jats:italic>Amphibiibacter pelophylacis<\/jats:italic> gen. nov., sp. nov.<\/jats:p>","DOI":"10.1099\/ijsem.0.006741","type":"journal-article","created":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T15:15:37Z","timestamp":1744211737000},"source":"Crossref","is-referenced-by-count":0,"title":["Phylogenomics as baseline for taxonomy description: Amphibiibacter pelophylacis gen. nov., sp. nov., a novel taxon of the family Sphaerotilaceae, class Betaproteobacteria, isolated from the skin microbiota of Pelophylax perezi from different populations"],"prefix":"10.1099","volume":"75","author":[{"given":"Sara","family":"Costa","sequence":"first","affiliation":[{"name":"CESAM & Department of Biology, Campus of Santiago, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1284-9082","authenticated-orcid":false,"given":"Diogo Neves","family":"Proen\u00e7a","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Coimbra, CEMMPRE, ARISE, 3000-456 Coimbra, Portugal"},{"name":"Present address: MED \u2013 Mediterranean Institute for Agriculture, Environment and Development & CHANGE \u2013 Global Change and Sustainability Institute, Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Isabel","family":"Lopes","sequence":"additional","affiliation":[{"name":"CESAM & Department of Biology, Campus of Santiago, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Paula V.","family":"Morais","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Coimbra, CEMMPRE, ARISE, 3000-456 Coimbra, Portugal"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-005-0009-1"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010957"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2007.110"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7429.2011.00787.x"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02060"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-017-1095-7"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph18052585"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-021-01215-6"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2016.07.230"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-017-6519-z"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004363"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-49-2-769"},{"key":"R13","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.syapm.2005.02.008","article-title":"Caldimonas taiwanensis sp. nov., a amylase producing bacterium isolated from a hot spring","volume":"28","author":"Chen","year":"2005","journal-title":"Syst Appl Microbiol"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.4518"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1038\/s43705-021-00047-7"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2022.126352"},{"key":"R17","article-title":"Field-sampling protocol for Batrachochytrium dendrobatidis from living amphibians, using alcohol preserved swabs. 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