{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T21:44:24Z","timestamp":1775511864489,"version":"3.50.1"},"reference-count":25,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,10,1]]},"abstract":"<jats:p>The composition of the essential oil of <jats:italic>Thymus pulegioides<\/jats:italic> and its antifungal activity on <jats:italic>Candida<\/jats:italic>, <jats:italic>Aspergillus<\/jats:italic> and dermatophyte fungal strains were studied. Essential oil from the aerial parts of the plant was obtained by hydrodistillation and analysed by GC and GC-MS. The oil showed high contents of carvacrol and thymol. The MIC and minimal lethal concentration were used to evaluate the antifungal activity against <jats:italic>Candida<\/jats:italic> (seven clinical isolates and four ATCC type strains), <jats:italic>Aspergillus<\/jats:italic> [five clinical isolates, and two Colecci\u00f3n Espa\u00f1ola de Cultivos Tipo (CECT) and two ATCC type strains] and five clinical dermatophyte strains. Antifungal activity was evaluated for the essential oil and for its main components. To clarify its mechanism of action on yeasts and filamentous fungi, flow-cytometric studies of cytoplasmic membrane integrity were performed, and the effect on the amount of ergosterol was investigated. Results showed that <jats:italic>T. pulegioides<\/jats:italic> essential oil exhibited a significant activity against clinically relevant fungi, mainly due to lesion formation in the cytoplasmic membrane and a considerable reduction of the ergosterol content. The present study indicates that <jats:italic>T. pulegioides<\/jats:italic> essential oil has considerable antifungal activity, deserving further investigation for clinical applications.<\/jats:p>","DOI":"10.1099\/jmm.0.46443-0","type":"journal-article","created":{"date-parts":[[2006,9,27]],"date-time":"2006-09-27T17:08:20Z","timestamp":1159376900000},"page":"1367-1373","source":"Crossref","is-referenced-by-count":226,"title":["Antifungal activity of the essential oil of Thymus pulegioides on Candida, Aspergillus and dermatophyte species"],"prefix":"10.1099","volume":"55","author":[{"given":"Eug\u00e9nia","family":"Pinto","sequence":"first","affiliation":[{"name":"Department of Microbiology\/CEQOFF, Faculty of Pharmacy, University of Porto, 4050-Porto, Portugal"}]},{"given":"Cid\u00e1lia","family":"Pina-Vaz","sequence":"additional","affiliation":[{"name":"IPATIMUP \u2013 Institute of Pathology and Molecular Immunology, University of Porto, 4200-Porto, Portugal"},{"name":"Department of 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