{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T03:55:52Z","timestamp":1777607752115,"version":"3.51.4"},"reference-count":57,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,2,1]]},"abstract":"<jats:p>In order to assess the potential of several molecular targets for the identification, typing and traceability of cyanobacteria in freshwater reservoirs, molecular techniques were applied to 118 cyanobacterial isolates mostly sourced from Portuguese freshwater reservoirs and representative of three orders of cyanobacteria: Chroococcales (54), Oscillatoriales (15) and Nostocales (49). The isolates were previously identified by morphological methods and subsequently characterized by composite hierarchical cluster analysis of STRR and LTRR (short and long tandemly repeated repetitive sequences) PCR fingerprinting profiles. Representative isolates were selected from each cluster and their molecular identification, at the species level, was obtained or confirmed by phylogenetic positioning using 16S rRNA gene and<jats:italic>rpoC1<\/jats:italic>phylogenies. A highly congruent association was observed between STTR- and LTRR-based clusters and taxonomic affiliation, revealing the usefulness of such PCR fingerprinting profiles for the identification of cyanobacteria. Composite analysis of hierarchical clustering of M13 and ERIC PCR fingerprints also appeared suitable for strain typing and traceability within a reservoir, indicating its potential for use in cyanobacterial monitoring, as a quality management control. Based on Simpson (<jats:italic>D<\/jats:italic>) and Shannon\u2013Wiener (<jats:italic>J<\/jats:italic>\u2032) indices a high diversity was observed within all species, with<jats:italic>Planktothrix agardhii<\/jats:italic>showing the lowest diversity values (<jats:italic>D<\/jats:italic>=0.83;<jats:italic>J<\/jats:italic>\u2032=0.88) and<jats:italic>Aphanizomenon flos-aquae<\/jats:italic>the highest ones (<jats:italic>D<\/jats:italic>=<jats:italic>J<\/jats:italic>\u2032=0.99). A diagnostic key based on 16S-ARDRA, ITS amplification and ITS-ARDRA for identification purposes is also presented.<\/jats:p>","DOI":"10.1099\/mic.0.022848-0","type":"journal-article","created":{"date-parts":[[2009,2,7]],"date-time":"2009-02-07T08:31:54Z","timestamp":1233995514000},"page":"642-656","source":"Crossref","is-referenced-by-count":70,"title":["Molecular identification, typing and traceability of cyanobacteria from freshwater reservoirs"],"prefix":"10.1099","volume":"155","author":[{"given":"Elisabete","family":"Val\u00e9rio","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia e Ecotoxicologia, Instituto Nacional de Sa\u00fade Dr Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisboa, Portugal"},{"name":"Universidade de Lisboa, Faculdade de Ci\u00eancias, Centro de Biodiversidade, Gen\u00f3mica Integrativa e Funcional (BioFIG), Edif\u00edcio ICAT, Campus da FCUL, Campo Grande, 1749-016 Lisboa, Portugal"}]},{"given":"L\u00e9lia","family":"Chambel","sequence":"additional","affiliation":[{"name":"Universidade de Lisboa, Faculdade de Ci\u00eancias, Centro de Biodiversidade, Gen\u00f3mica Integrativa e Funcional (BioFIG), Edif\u00edcio ICAT, Campus da FCUL, Campo Grande, 1749-016 Lisboa, Portugal"}]},{"given":"S\u00e9rgio","family":"Paulino","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia e Ecotoxicologia, Instituto Nacional de Sa\u00fade Dr Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisboa, Portugal"}]},{"given":"Nat\u00e1lia","family":"Faria","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia e Ecotoxicologia, Instituto Nacional de Sa\u00fade Dr Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisboa, Portugal"}]},{"given":"Paulo","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia e Ecotoxicologia, Instituto Nacional de Sa\u00fade Dr Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisboa, Portugal"}]},{"given":"Rog\u00e9rio","family":"Tenreiro","sequence":"additional","affiliation":[{"name":"Universidade de Lisboa, Faculdade de Ci\u00eancias, Centro de Biodiversidade, Gen\u00f3mica Integrativa e Funcional (BioFIG), Edif\u00edcio ICAT, Campus da FCUL, Campo Grande, 1749-016 Lisboa, Portugal"}]}],"member":"345","reference":[{"issue":"29","key":"R1","article-title":"Identification of Common Noxious Cyanobacteria Part I \u2013 Nostocales : Urban Water Research Association of Australia","volume":"no","author":"Baker","year":"1991","journal-title":"Research Report"},{"issue":"46","key":"R2","article-title":"Identification of Common Noxious Cyanobacteria Part II \u2013 Chroococales Oscillatoriales : Urban Water Research Association of Australia","volume":"no","author":"Baker","year":"1992","journal-title":"Research Report"},{"key":"R3","volume-title":"Les Algues d'Eau Douce: Les Algues Bleus et Rouges, Les Eugle\u0301niens, Peridiniens, et Cryptomonadines","author":"Bourrelly","year":"1970"},{"key":"R4","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1080\/01490450600760732","article-title":"Genetic characterization of epilithic cyanobacteria and their associated bacteria","volume":"23","author":"Bruno","year":"2006","journal-title":"Geomicrobiol J"},{"key":"R5","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1007\/978-0-387-21609-6_27","article-title":"Phylum BX. 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