{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T03:38:14Z","timestamp":1770349094526,"version":"3.49.0"},"reference-count":64,"publisher":"Microbiology Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,1,1]]},"abstract":"<jats:p>PvdQ, an acylase from<jats:italic>Pseudomonas aeruginosa<\/jats:italic>PAO1, has been shown to have at least two functions. It can act as a quorum quencher due to its ability to degrade long-chain<jats:italic>N<\/jats:italic>-acylhomoserine lactones (AHLs), e.g. 3-oxo-C12-HSL, leading to a decrease in virulence factors. In addition, PvdQ is involved in iron homeostasis by playing a role in the biosynthesis of pyoverdine, the major siderophore of<jats:italic>P. aeruginosa<\/jats:italic>. In accordance with earlier studies on RNA level, we could show at the protein level that PvdQ is only expressed when iron is present at very low concentrations. We therefore set out to investigate the two functions of PvdQ under iron-limiting conditions. Gene deletion of<jats:italic>pvdQ<\/jats:italic>does not affect growth of<jats:italic>P. aeruginosa<\/jats:italic>but abrogates pyoverdine production, and results in an accumulation of 3-oxo-C12-HSL. Phenotypic analyses of our \u0394<jats:italic>pvdQ<\/jats:italic>mutant at low iron concentrations revealed that this mutant is impaired in swarming motility and biofilm formation. Additionally, a plant and a<jats:italic>Caenorhabditis elegans<\/jats:italic>infection model demonstrated that the deletion of<jats:italic>pvdQ<\/jats:italic>resulted in reduced virulence. None of the phenotypes in the present study could be linked to the presence or absence of AHLs. These results clearly indicate that under iron-limiting conditions PvdQ plays a major role in swarming motility, in biofilm development and in infection that is more likely to be linked to the pyoverdine pathway rather than the LasI\/LasR\/3-oxo-C12-HSL quorum-sensing circuit.<\/jats:p>","DOI":"10.1099\/mic.0.030973-0","type":"journal-article","created":{"date-parts":[[2009,11,3]],"date-time":"2009-11-03T22:52:21Z","timestamp":1257288741000},"page":"49-59","source":"Crossref","is-referenced-by-count":94,"title":["Role of PvdQ in Pseudomonas aeruginosa virulence under iron-limiting conditions"],"prefix":"10.1099","volume":"156","author":[{"given":"Pol Nadal","family":"Jimenez","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Biology, University of Groningen, 9713 AV Groningen, The Netherlands"}]},{"given":"Gudrun","family":"Koch","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology, University of Groningen, 9713 AV Groningen, The 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