{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T15:03:29Z","timestamp":1768748609665,"version":"3.49.0"},"reference-count":32,"publisher":"Microbiology Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,1,1]]},"abstract":"<jats:p>Transcription of genes coding for formate dehydrogenases (<jats:italic>fdh<\/jats:italic>genes) and hydrogenases (<jats:italic>hyd<\/jats:italic>genes) in<jats:italic>Syntrophobacter fumaroxidans<\/jats:italic>and<jats:italic>Methanospirillum hungatei<\/jats:italic>was studied following growth under different conditions. Under all conditions tested, all<jats:italic>fdh<\/jats:italic>and<jats:italic>hyd<\/jats:italic>genes were transcribed. However, transcription levels of the individual genes varied depending on the substrate and growth conditions. Our results strongly suggest that in syntrophically grown<jats:italic>S. fumaroxidans<\/jats:italic>cells, the [FeFe]-hydrogenase (encoded by Sfum_844-46), FDH1 (Sfum_2703-06) and Hox (Sfum_2713-16) may confurcate electrons from NADH and ferredoxin to protons and carbon dioxide to produce hydrogen and formate, respectively. Based on bioinformatic analysis, a membrane-integrated energy-converting [NiFe]-hydrogenase (Mhun_1741-46) of<jats:italic>M. hungatei<\/jats:italic>might be involved in the energy-dependent reduction of CO<jats:sub>2<\/jats:sub>to formylmethanofuran. The best candidates for F<jats:sub>420<\/jats:sub>-dependent<jats:italic>N<\/jats:italic><jats:sup>5<\/jats:sup>,<jats:italic>N<\/jats:italic><jats:sup>10<\/jats:sup>-methyl-H<jats:sub>4<\/jats:sub>MPT and<jats:italic>N<\/jats:italic><jats:sup>5<\/jats:sup>,<jats:italic>N<\/jats:italic><jats:sup>10<\/jats:sup>,-methylene-H<jats:sub>4<\/jats:sub>MPT reduction are the cytoplasmic [NiFe]-hydrogenase and FDH1. 16S rRNA ratios indicate that in one of the triplicate co-cultures of<jats:italic>S. fumaroxidans<\/jats:italic>and<jats:italic>M. hungatei<\/jats:italic>, less energy was available for<jats:italic>S. fumaroxidans.<\/jats:italic>This led to enhanced transcription of genes coding for the Rnf-complex (Sfum_2694-99) and of several<jats:italic>fdh<\/jats:italic>and<jats:italic>hyd<\/jats:italic>genes. The Rnf-complex probably reoxidized NADH with ferredoxin reduction, followed by ferredoxin oxidation by the induced formate dehydrogenases and hydrogenases.<\/jats:p>","DOI":"10.1099\/mic.0.043927-0","type":"journal-article","created":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:56:19Z","timestamp":1285894579000},"page":"280-289","source":"Crossref","is-referenced-by-count":63,"title":["Growth- and substrate-dependent transcription of formate dehydrogenase and hydrogenase coding genes in Syntrophobacter fumaroxidans and Methanospirillum hungatei"],"prefix":"10.1099","volume":"157","author":[{"given":"Petra","family":"Worm","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands"}]},{"given":"Alfons J. 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