{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T11:33:07Z","timestamp":1767007987670,"version":"3.37.3"},"reference-count":49,"publisher":"Microbiology Society","issue":"7","funder":[{"DOI":"10.13039\/501100000268","name":"BBSRC","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"name":"MRC"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,7,1]]},"abstract":"<jats:p>The ability of serovars of <jats:italic>Salmonella enterica<\/jats:italic> to cause systemic disease is dependent upon their survival and replication within macrophages. To do this, bacteria must withstand or surmount bacteriostatic and bactericidal responses by the host cell, including the delivery of hydrolytic enzymes from lysosomes to the phagosome. The bacterial two-component regulatory system PhoP\/Q has been implicated in avoidance of phagolysosomal fusion by <jats:italic>S. enterica<\/jats:italic> serovar Typhimurium (<jats:italic>S.<\/jats:italic> Typhimurium) in murine macrophages. In this study, the involvement of PhoP\/Q-activated genes in avoidance of phagolysosomal fusion was analysed: of all the <jats:italic>S.<\/jats:italic> Typhimurium mutant strains tested, only an <jats:italic>mgtC<\/jats:italic> mutant strain partially reproduced the phenotype of the <jats:italic>phoP<\/jats:italic> mutant strain. As this gene is required for bacterial growth in magnesium-depleted conditions <jats:italic>in vitro<\/jats:italic>, the contributions of PhoP\/Q to intramacrophage replication and survival were reappraised. Although PhoP\/Q was required for both replication and survival of <jats:italic>S.<\/jats:italic> Typhimurium within murine macrophages, subsequent analysis of the kinetics of phagolysosomal fusion, taking account of differences in the replication rates of wild-type and <jats:italic>phoP<\/jats:italic> mutant strains, provided no evidence for a PhoP\/Q-dependent role in this process. PhoP\/Q appeared to act subsequent to the process of phagolysosomal avoidance and to promote replication of those bacteria that had already escaped a phagolysosomal fate. Therefore, we conclude that the PhoP\/Q regulon enables <jats:italic>S<\/jats:italic>. Typhimurium to adapt to intramacrophage stresses other than phagolysosomal fusion.<\/jats:p>","DOI":"10.1099\/mic.0.048926-0","type":"journal-article","created":{"date-parts":[[2011,4,22]],"date-time":"2011-04-22T00:56:17Z","timestamp":1303433777000},"page":"2084-2093","source":"Crossref","is-referenced-by-count":50,"title":["Contribution of the PhoP\/Q regulon to survival and replication of Salmonella enterica serovar Typhimurium in macrophages"],"prefix":"10.1099","volume":"157","author":[{"given":"Jessica A.","family":"Thompson","sequence":"first","affiliation":[{"name":"Section of Microbiology, Centre for Molecular Microbiology and Infection, Imperial College London, Armstrong Road, London SW7 2AZ, UK"}]},{"given":"Mei","family":"Liu","sequence":"additional","affiliation":[{"name":"Section of Microbiology, Centre for Molecular Microbiology and Infection, Imperial College London, Armstrong Road, London SW7 2AZ, UK"}]},{"given":"Sophie","family":"Helaine","sequence":"additional","affiliation":[{"name":"Section of Microbiology, Centre for Molecular Microbiology and Infection, Imperial College London, Armstrong Road, London SW7 2AZ, UK"}]},{"given":"David W.","family":"Holden","sequence":"additional","affiliation":[{"name":"Section of Microbiology, Centre for Molecular Microbiology and Infection, Imperial College London, Armstrong Road, London SW7 2AZ, UK"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"2409","DOI":"10.1128\/JB.180.9.2409-2417.1998","article-title":"A low pH-inducible, PhoPQ-dependent acid tolerance response protects Salmonella typhimurium against inorganic acid stress","volume":"180","author":"Bearson","year":"1998","journal-title":"J Bacteriol"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1007295107"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01527.x"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.13.3235"},{"key":"r5","doi-asserted-by":"crossref","first-page":"2705","DOI":"10.1099\/00221287-148-9-2705","article-title":"Growth and killing of a Salmonella enterica serovar Typhimurium sifA mutant strain in the cytosol of different host cell lines","volume":"148","author":"Beuz\u00f3n","year":"2002","journal-title":"Microbiology"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.17.5376"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.12.3203-3213.2002"},{"key":"r8","doi-asserted-by":"crossref","first-page":"2232","DOI":"10.1128\/IAI.59.7.2232-2238.1991","article-title":"Inhibition of macrophage phagosome-lysosome fusion by Salmonella typhimurium","volume":"59","author":"Buchmeier","year":"1991","journal-title":"Infect Immun"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004975"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.25.13997"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03455.x"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2005.00360.x"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.152.1.165"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00153.x"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200308038"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.18.7077"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00296-06"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81750-X"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.21.16281"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00144-09"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000041107"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1126\/science.7618105"},{"key":"r24","doi-asserted-by":"crossref","first-page":"3038","DOI":"10.1128\/IAI.61.7.3038-3046.1993","article-title":"Antimicrobial proteins of murine macrophages","volume":"61","author":"Hiemstra","year":"1993","journal-title":"Infect Immun"},{"key":"r25","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1002\/jlb.66.3.423","article-title":"Ubiquicidin, a novel murine microbicidal protein present in the cytosolic fraction of macrophages","volume":"66","author":"Hiemstra","year":"1999","journal-title":"J Leukoc Biol"},{"key":"r26","doi-asserted-by":"crossref","first-page":"4742","DOI":"10.1128\/JB.171.9.4742-4751.1989","article-title":"Magnesium transport in Salmonella typhimurium: genetic characterization and cloning of three magnesium transport loci","volume":"171","author":"Hmiel","year":"1989","journal-title":"J Bacteriol"},{"key":"r27","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00701.x"},{"key":"r28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00890.x"},{"key":"r29","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.2.237"},{"key":"r30","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.13.5054"},{"key":"r31","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/014290-0"},{"key":"r32","doi-asserted-by":"crossref","first-page":"3802","DOI":"10.1128\/IAI.66.8.3802-3809.1998","article-title":"Magnesium and the role of MgtC in growth of Salmonella typhimurium","volume":"66","author":"Moncrief","year":"1998","journal-title":"Infect Immun"},{"key":"r33","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.140.3.591"},{"key":"r34","doi-asserted-by":"crossref","first-page":"3137","DOI":"10.1091\/mbc.11.9.3137","article-title":"Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and is required for late endosome-lysosome fusion","volume":"11","author":"Mullock","year":"2000","journal-title":"Mol Biol Cell"},{"key":"r35","doi-asserted-by":"crossref","first-page":"5410","DOI":"10.1128\/IAI.64.12.5410-5412.1996","article-title":"Distribution of pathogenicity islands in Salmonella spp","volume":"64","author":"Ochman","year":"1996","journal-title":"Infect Immun"},{"key":"r36","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.149.5.1053"},{"key":"r37","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05542.x"},{"key":"r38","doi-asserted-by":"crossref","first-page":"2765","DOI":"10.1128\/IAI.64.7.2765-2773.1996","article-title":"Acidification of phagosomes containing Salmonella typhimurium in murine macrophages","volume":"64","author":"Rathman","year":"1996","journal-title":"Infect Immun"},{"key":"r39","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1128\/IAI.65.4.1475-1485.1997","article-title":"The unique trafficking pattern of Salmonella typhimurium-containing phagosomes in murine macrophages is independent of the mechanism of bacterial entry","volume":"65","author":"Rathman","year":"1997","journal-title":"Infect Immun"},{"key":"r40","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0304455101"},{"volume-title":"Molecular Cloning: a Laboratory Manual","year":"2001","author":"Sambrook","key":"r41"},{"key":"r42","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1005-1013.2000"},{"key":"r43","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1128\/IAI.67.1.213-219.1999","article-title":"Influence of the Salmonella typhimurium pathogenicity island 2 type III secretion system on bacterial growth in the mouse","volume":"67","author":"Shea","year":"1999","journal-title":"Infect Immun"},{"key":"r44","doi-asserted-by":"crossref","first-page":"5092","DOI":"10.1128\/JB.178.17.5092-5099.1996","article-title":"Molecular basis of the magnesium deprivation response in Salmonella typhimurium: identification of PhoP-regulated genes","volume":"178","author":"Soncini","year":"1996","journal-title":"J Bacteriol"},{"key":"r45","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)00856-1"},{"key":"r46","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02787.x"},{"key":"r47","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.14.3924"},{"key":"r48","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.2.227"},{"key":"r49","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5458.1655"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.048926-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,19]],"date-time":"2020-11-19T11:23:43Z","timestamp":1605785023000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.048926-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,7,1]]},"references-count":49,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2011,7,1]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.048926-0","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"type":"print","value":"1350-0872"},{"type":"electronic","value":"1465-2080"}],"subject":[],"published":{"date-parts":[[2011,7,1]]}}}