{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T06:57:32Z","timestamp":1761893852759},"reference-count":49,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,9,1]]},"abstract":"The Bacillus subtilis<\/jats:italic> proteins involved in the utilization of l<\/jats:sc>-arabinose are encoded by the araABDLMNPQ<\/jats:italic>\u2013abfA<\/jats:italic> metabolic operon and by the araE<\/jats:italic>\/araR<\/jats:italic> divergent unit. Transcription from the ara<\/jats:italic> operon, araE<\/jats:italic> transport gene and araR<\/jats:italic> regulatory gene is induced by l<\/jats:sc>-arabinose and negatively controlled by AraR. Additionally, expression of both the ara<\/jats:italic> operon and the araE<\/jats:italic> gene is regulated at the transcriptional level by glucose repression. Here, by transcriptional fusion analysis in different mutant backgrounds, it is shown that CcpA most probably complexed with HPr-Ser46-P plays the major role in carbon catabolite repression of the ara<\/jats:italic> regulon by glucose and glycerol. Site-directed mutagenesis and deletion analysis indicate that two catabolite responsive elements (cre<\/jats:italic>s) present in the ara<\/jats:italic> operon (cre araA<\/jats:italic> and cre araB<\/jats:italic>) and one cre<\/jats:italic> in the araE<\/jats:italic> gene (cre araE<\/jats:italic>) are implicated in this mechanism. Furthermore, cre araA<\/jats:italic> located between the promoter region of the ara<\/jats:italic> operon and the araA<\/jats:italic> gene, and cre araB<\/jats:italic> placed 2\u2005kb downstream within the araB<\/jats:italic> gene are independently functional and both contribute to glucose repression. In Northern blot analysis, in the presence of glucose, a CcpA-dependent transcript consistent with a message stopping at cre araB<\/jats:italic> was detected, suggesting that transcription \u2018roadblocking\u2019 of RNA polymerase elongation is the most likely mechanism operating in this system. Glucose exerts an additional repression of the ara<\/jats:italic> regulon, which requires a functional araR<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/mic.0.26326-0","type":"journal-article","created":{"date-parts":[[2003,8,29]],"date-time":"2003-08-29T23:35:15Z","timestamp":1062200115000},"page":"2345-2355","source":"Crossref","is-referenced-by-count":26,"title":["Distinct molecular mechanisms involved in carbon catabolite repression of the arabinose regulon in Bacillus subtilis"],"prefix":"10.1099","volume":"149","author":[{"given":"Jos\u00e9 Manuel","family":"In\u00e1cio","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Avenida de Rep\u00fablica, Apartado 127, 2781-901 Oeiras, Portugal"}]},{"given":"Carla","family":"Costa","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Avenida de Rep\u00fablica, Apartado 127, 2781-901 Oeiras, Portugal"}]},{"given":"Isabel","family":"de S\u00e1-Nogueira","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, 2829-516 Caparica, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Avenida de Rep\u00fablica, Apartado 127, 2781-901 Oeiras, Portugal"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"2497","DOI":"10.1128\/JB.183.8.2497-2504.2001","article-title":"Regulation of the acetoin catabolic pathway is controlled by sigma L in Bacillus subtilis","volume":"183","author":"Ali","year":"2001","journal-title":"J Bacteriol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0022-2836(02)00245-0","article-title":"Quantification of the influence of HPrSer46P on CcpA- cre interaction","volume":"319","author":"Aung-Hilbrich","year":"2002","journal-title":"J Mol 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