{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T15:52:53Z","timestamp":1759938773248},"reference-count":20,"publisher":"Microbiology Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,7,1]]},"abstract":"<jats:p>The basis of fructophily in the yeast<jats:italic>Zygosaccharomyces bailii<\/jats:italic>has been shown to reside in the performance of transport systems for hexoses. In this study, a gene encoding a fructose-specific transporter was characterized. The strategy involved the functional complementation of a<jats:italic>Saccharomyces cerevisiae<\/jats:italic>strain that does not take up hexoses (<jats:italic>hxt-null<\/jats:italic>strain). This strain was transformed with a genomic library of<jats:italic>Z. bailii<\/jats:italic>. One transformant capable of growing on fructose, but not on glucose, was obtained. This transformant did not transport<jats:sc>d<\/jats:sc>-[<jats:sup>14<\/jats:sup>C]glucose, and the kinetic parameters for<jats:sc>d<\/jats:sc>-[<jats:sup>14<\/jats:sup>C]fructose were<jats:italic>V<\/jats:italic><jats:sub>max<\/jats:sub>=3\u00b73\u2005mmol\u2005h<jats:sup>\u22121<\/jats:sup>\u2005g<jats:sup>\u22121<\/jats:sup>and<jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub>=80\u00b74\u2005mM. As in the original strain of<jats:italic>Z. bailii<\/jats:italic>, fructose uptake was not inhibited by the presence of other hexoses or uranyl. The plasmid responsible for the observed phenotype was found to carry an ORF encoding a 616\u2005amino acid protein with the characteristics of a membrane transporter, which was designated<jats:italic>FFZ1<\/jats:italic>(fructose facilitator<jats:italic>Zygosaccharomyces<\/jats:italic>). The impairment in function observed in an<jats:italic>S. cerevisiae<\/jats:italic>transformant expressing a truncated Ffz1 protein lacking 67\u2005amino acids at the C-terminus suggests an important role for this terminal part in the proper structure of the transporter.<\/jats:p>","DOI":"10.1099\/mic.0.26979-0","type":"journal-article","created":{"date-parts":[[2004,7,15]],"date-time":"2004-07-15T17:45:21Z","timestamp":1089913521000},"page":"2429-2433","source":"Crossref","is-referenced-by-count":51,"title":["Ffz1, a new transporter specific for fructose from Zygosaccharomyces bailii"],"prefix":"10.1099","volume":"150","author":[{"given":"Carlos","family":"Pina","sequence":"first","affiliation":[{"name":"Dep. 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