{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T07:44:40Z","timestamp":1774770280211,"version":"3.50.1"},"reference-count":62,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,6,1]]},"abstract":"<jats:p><jats:italic>Mycobacterium smegmatis<\/jats:italic>is often used as a surrogate host for pathogenic mycobacteria, especially since the isolation of the transformable smooth morphotype strain mc<jats:sup>2<\/jats:sup>155 from the isogenic rough wild-type strain ATCC 607. Biochemical analysis of the cell envelope components revealed a lack of polar glycolipids, namely the lipooligosaccharides and the polar subfamilies of glycopeptidolipids, in the mc<jats:sup>2<\/jats:sup>155 strain. In addition, the latter strain differs from its parent by the distribution of various species of glycolipids and phospholipids between the outermost and deeper layers of the cell envelope. The presence of filamentous and rope-like structures at the cell surface of mc<jats:sup>2<\/jats:sup>155 cells grown in complex media further supported an ultrastructural change in the cell envelope of the mutant. Importantly, a significantly more rapid uptake of the hydrophobic chenodeoxycholate was observed for the mutant compared to wild-type cells. Taken together, these data indicate that the nature of the surface-exposed and envelope constituents is crucial for the surface properties, cell wall permeability and bacterial phenotype, and suggest that the transformable character of the mc<jats:sup>2<\/jats:sup>155 strain may be in part explained by these profound modifications of its cell envelope.<\/jats:p>","DOI":"10.1099\/mic.0.27869-0","type":"journal-article","created":{"date-parts":[[2005,6,7]],"date-time":"2005-06-07T19:33:51Z","timestamp":1118172831000},"page":"2075-2086","source":"Crossref","is-referenced-by-count":76,"title":["The cell envelope structure and properties of Mycobacterium smegmatis mc2155: is there a clue for the unique transformability of the strain?"],"prefix":"10.1099","volume":"151","author":[{"given":"Gilles","family":"Etienne","sequence":"first","affiliation":[{"name":"Institut de Pharmacologie et de Biologie Structurale, Unit\u00e9 Mixte de Recherche du Centre National de la Recherche Scientifique et de l'Universit\u00e9 Paul Sabatier (UMR5089), D\u00e9partement \u2018M\u00e9canismes Mol\u00e9culaires des Infections Mycobact\u00e9riennes\u2019, 205 route de Narbonne, 31077 Toulouse cedex 04, France"}]},{"given":"Fran\u00e7oise","family":"Laval","sequence":"additional","affiliation":[{"name":"Institut de Pharmacologie et de Biologie Structurale, Unit\u00e9 Mixte de Recherche du Centre National de la Recherche Scientifique et de l'Universit\u00e9 Paul Sabatier (UMR5089), D\u00e9partement \u2018M\u00e9canismes Mol\u00e9culaires des Infections Mycobact\u00e9riennes\u2019, 205 route de Narbonne, 31077 Toulouse cedex 04, France"}]},{"given":"Christelle","family":"Villeneuve","sequence":"additional","affiliation":[{"name":"Institut de Pharmacologie et de Biologie Structurale, Unit\u00e9 Mixte de Recherche du Centre National de la Recherche Scientifique et de l'Universit\u00e9 Paul Sabatier (UMR5089), D\u00e9partement \u2018M\u00e9canismes Mol\u00e9culaires des Infections Mycobact\u00e9riennes\u2019, 205 route de Narbonne, 31077 Toulouse cedex 04, France"}]},{"given":"Premkumar","family":"Dinadayala","sequence":"additional","affiliation":[{"name":"Institut de Pharmacologie et de Biologie Structurale, Unit\u00e9 Mixte de Recherche du Centre National de la Recherche Scientifique et de l'Universit\u00e9 Paul Sabatier (UMR5089), D\u00e9partement \u2018M\u00e9canismes Mol\u00e9culaires des Infections Mycobact\u00e9riennes\u2019, 205 route de Narbonne, 31077 Toulouse cedex 04, France"}]},{"given":"Ahmed","family":"Abouwarda","sequence":"additional","affiliation":[{"name":"Institut de Pharmacologie et de Biologie Structurale, Unit\u00e9 Mixte de Recherche du Centre National de la Recherche Scientifique et de l'Universit\u00e9 Paul Sabatier (UMR5089), D\u00e9partement \u2018M\u00e9canismes Mol\u00e9culaires des Infections Mycobact\u00e9riennes\u2019, 205 route de Narbonne, 31077 Toulouse cedex 04, France"}]},{"given":"Didier","family":"Zerbib","sequence":"additional","affiliation":[{"name":"Institut de Pharmacologie et de Biologie Structurale, Unit\u00e9 Mixte de Recherche du Centre National de la Recherche Scientifique et de l'Universit\u00e9 Paul Sabatier (UMR5089), D\u00e9partement \u2018M\u00e9canismes Mol\u00e9culaires des Infections Mycobact\u00e9riennes\u2019, 205 route de Narbonne, 31077 Toulouse cedex 04, France"}]},{"given":"Alexandra","family":"Galamba","sequence":"additional","affiliation":[{"name":"Unidade de Micobact\u00e9rias, Instituto Nacional de Sa\u00fade Dr Ricardo Jorge, Av. 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Structures and biological activities of the cell envelope glycoconjugates","volume-title":"Glycomicrobiology","author":"Daffe\u0301","year":"2000"},{"key":"R18","first-page":"579","article-title":"Inte\u0301re\u0302t taxonomique des acides gras des mycobacte\u0301ries: proposition d'une me\u0301thode d'analyse","volume":"134B","author":"Daffe\u0301","year":"1983a","journal-title":"Ann Microbiol"},{"key":"R19","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/0005-2760(83)90304-1","article-title":"Structural elucidation by field desorption and electron-impact mass spectrometry of the C-mycosides isolated from Mycobacterium smegmatis","volume":"751","author":"Daffe\u0301","year":"1983b","journal-title":"Biochim Biophys Acta"},{"key":"R20","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/j.1574-6968.1989.tb03558.x","article-title":"The cell envelope of Mycobacterium smegmatis: cytochemistry and architectural implications","volume":"61","author":"Daffe\u0301","year":"1989","journal-title":"FEMS Microbiol Lett"},{"key":"R21","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1021\/bi00216a011","article-title":"Novel type-specific lipoologosaccharides from Mycobacterium tuberculosis","volume":"30","author":"Daffe\u0301","year":"1991","journal-title":"Biochemistry"},{"key":"R22","first-page":"488","article-title":"Color reaction of hexoses","volume":"1","author":"Dische","year":"1962","journal-title":"Methods Carbohydr Chem"},{"issue":"1","key":"R23","first-page":"9","article-title":"The anatomy of mycobacteria","volume-title":"The Biology of the Mycobacteria","volume":"vol","author":"Draper","year":"1982"},{"key":"R24","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1046\/j.1365-2958.2000.01882.x","article-title":"Oxygenated mycolic acids are necessary for virulence of Mycobacterium tuberculosis in mice","volume":"36","author":"Dubnau","year":"2000","journal-title":"Mol Microbiol"},{"key":"R25","doi-asserted-by":"crossref","first-page":"6177","DOI":"10.1128\/JB.182.21.6177-6182.2000","article-title":"A genetic mechanism for deletion of the ser2 gene cluster and formation of rough morphological variants ofMycobacterium avium","volume":"182","author":"Eckstein","year":"2000","journal-title":"J Bacteriol"},{"key":"R26","doi-asserted-by":"crossref","first-page":"3089","DOI":"10.1099\/00221287-148-10-3089","article-title":"The impact of the absence of glycopeptidolipids on the ultrastructure, cell surface and cell wall properties, and phagocytosis of Mycobacterium smegmatis","volume":"148","author":"Etienne","year":"2002","journal-title":"Microbiology"},{"key":"R27","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1128\/JB.111.2.404-411.1972","article-title":"Nature of the receptor substance of Mycobacterium smegmatis for D4 bacteriophage adsorption","volume":"111","author":"Furuchi","year":"1972","journal-title":"J Bacteriol"},{"key":"R28","doi-asserted-by":"crossref","first-page":"3281","DOI":"10.1099\/00221287-147-12-3281","article-title":"Disruption of adhC reveals a large duplication in the Mycobacterium smegmatis mc2155 genome","volume":"147","author":"Galamba","year":"2001","journal-title":"Microbiology"},{"key":"R29","doi-asserted-by":"crossref","first-page":"27292","DOI":"10.1074\/jbc.270.45.27292","article-title":"The biosynthesis of cyclopropanated mycolic acids in Mycobacterium tuberculosis. 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