{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T02:25:42Z","timestamp":1768098342806,"version":"3.49.0"},"reference-count":44,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,9,1]]},"abstract":"<jats:p>In the microbial competition observed in enhanced biological phosphorus removal (EBPR) systems, an undesirable group of micro-organisms known as glycogen-accumulating organisms (GAOs) compete for carbon in the anaerobic period with the desired polyphosphate-accumulating organisms (PAOs). Some studies have suggested that a propionate carbon source provides PAOs with a competitive advantage over GAOs in EBPR systems; however, the metabolism of GAOs with this carbon source has not been previously investigated. In this study, GAOs were enriched in a laboratory-scale bioreactor with propionate as the sole carbon source, in an effort to better understand their biochemical processes. Based on comprehensive solid-, liquid- and gas-phase chemical analytical data from the bioreactor, a metabolic model was proposed for the metabolism of propionate by GAOs. The model adequately described the anaerobic stoichiometry observed through chemical analysis, and can be a valuable tool for further investigation of the competition between PAOs and GAOs, and for the optimization of the EBPR process. A group of Alphaproteobacteria dominated the biomass (96\u200a% of Bacteria) from this bioreactor, while post-fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH) chemical staining confirmed that these Alphaproteobacteria produced poly-<jats:italic>\u03b2<\/jats:italic>-hydroxyalkanoates (PHAs) anaerobically and utilized them aerobically, demonstrating that they were putative GAOs. Some of the Alphaproteobacteria were related to <jats:italic>Defluvicoccus vanus<\/jats:italic> (16\u200a% of Bacteria), but the specific identity of many could not be determined by FISH. Further investigation into the identity of other GAOs is necessary.<\/jats:p>","DOI":"10.1099\/mic.0.28065-0","type":"journal-article","created":{"date-parts":[[2006,8,31]],"date-time":"2006-08-31T20:32:03Z","timestamp":1157056323000},"page":"2767-2778","source":"Crossref","is-referenced-by-count":106,"title":["Anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source"],"prefix":"10.1099","volume":"152","author":[{"given":"Adrian","family":"Oehmen","sequence":"first","affiliation":[{"name":"Advanced Wastewater Management Centre (AWMC), The University of Queensland, St Lucia, Brisbane 4072, Australia"}]},{"given":"Raymond J.","family":"Zeng","sequence":"additional","affiliation":[{"name":"Advanced Wastewater Management Centre (AWMC), The University of Queensland, St Lucia, Brisbane 4072, Australia"}]},{"given":"Aaron M.","family":"Saunders","sequence":"additional","affiliation":[{"name":"Advanced Wastewater Management Centre (AWMC), The University of Queensland, St Lucia, Brisbane 4072, Australia"}]},{"given":"Linda L.","family":"Blackall","sequence":"additional","affiliation":[{"name":"Advanced Wastewater Management Centre (AWMC), The University of Queensland, St Lucia, Brisbane 4072, Australia"}]},{"given":"J\u00fcrg","family":"Keller","sequence":"additional","affiliation":[{"name":"Advanced Wastewater Management Centre (AWMC), The University of Queensland, St Lucia, Brisbane 4072, Australia"}]},{"given":"Zhiguo","family":"Yuan","sequence":"additional","affiliation":[{"name":"Advanced Wastewater Management Centre (AWMC), The University of Queensland, St Lucia, Brisbane 4072, Australia"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-94-011-0351-0_23","article-title":"In situ identification of microorganisms by whole cell hybridization with rRNA-targeted nucleic acid probes","volume-title":"Molecular Microbial Ecology 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