{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T16:11:41Z","timestamp":1764173501646},"reference-count":42,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,12,1]]},"abstract":"<jats:p>Herpes simplex virus type 1 (HSV-1) has the capacity to establish a life-long latent infection in sensory neurones and also to periodically reactivate from these cells. Since mutant viruses defective for immediate-early (IE) expression retain the capacity for latency establishment it is widely assumed that latency is the consequence of a block in IE gene expression. However, it is not clear whether viral gene expression can precede latency establishment following wild-type virus infection. In order to address this question we have utilized a reporter mouse model system to facilitate a historical analysis of viral promoter activation <jats:italic>in vivo<\/jats:italic>. This system utilizes recombinant viruses expressing Cre recombinase under the control of different viral promoters and the Cre reporter mouse strain ROSA26R. In this model, viral promoter-driven Cre recombinase mediates a permanent genetic change, resulting in reporter gene activation and permanent marking of latently infected cells. The analyses of HSV-1 recombinants containing human cytomegalovirus major immediate-early, ICP0, gC or latency-associated transcript promoters linked to Cre recombinase in this system have revealed the existence of a population of neurones that have experienced IE promoter activation prior to the establishment of latency.<\/jats:p>","DOI":"10.1099\/vir.0.2008\/005066-0","type":"journal-article","created":{"date-parts":[[2008,11,13]],"date-time":"2008-11-13T19:43:46Z","timestamp":1226605426000},"page":"2965-2974","source":"Crossref","is-referenced-by-count":59,"title":["A historical analysis of herpes simplex virus promoter activation in vivo reveals distinct populations of latently infected neurones"],"prefix":"10.1099","volume":"89","author":[{"given":"Jo\u00e3o T.","family":"Proen\u00e7a","sequence":"first","affiliation":[{"name":"Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK"}]},{"given":"Heather 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