{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T04:18:47Z","timestamp":1774930727723,"version":"3.50.1"},"reference-count":26,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,9,1]]},"abstract":"<jats:p>To understand the mechanisms involved in protective immunity to African swine fever virus (ASFV) infection, the observation that infection with the avirulent Portuguese ASFV isolate OUR\/T88\/3 protects outbred pigs from challenge with the virulent Portuguese ASFV isolate OUR\/T88\/1 was exploited. It was demonstrated that pigs exposed to OUR\/T88\/3 and then depleted of CD8<jats:sup>+<\/jats:sup>lymphocytes were no longer fully protected from OUR\/T88\/1 challenge. This indicated that CD8<jats:sup>+<\/jats:sup>lymphocytes play an important role in the protective immune response to ASFV infection and that anti-ASFV antibody alone, from OUR\/T88\/3 infection, was not sufficient to protect pigs from OUR\/T88\/1 challenge. Inbred pigs of the cc haplotype infected with OUR\/T88\/3 were not always protected from OUR\/T88\/1 challenge and developed both viraemia and fever. Such viraemia was always correlated with increased numbers of circulating CD8<jats:italic>\u03b2<\/jats:italic><jats:sup>+<\/jats:sup>lymphocytes, indicating a specific role for CD8<jats:italic>\u03b2<\/jats:italic><jats:sup>+<\/jats:sup>lymphocytes in combating viraemia. These experiments indicate an important role for CD8<jats:sup>+<\/jats:sup>lymphocytes, particularly CD8<jats:italic>\u03b2<\/jats:italic><jats:sup>+<\/jats:sup>lymphocytes, in ASF protective immunity.<\/jats:p>","DOI":"10.1099\/vir.0.81038-0","type":"journal-article","created":{"date-parts":[[2005,8,12]],"date-time":"2005-08-12T00:40:29Z","timestamp":1123807229000},"page":"2445-2450","source":"Crossref","is-referenced-by-count":215,"title":["In vivo depletion of CD8+ T lymphocytes abrogates protective immunity to African swine fever virus"],"prefix":"10.1099","volume":"86","author":[{"given":"C. A. L.","family":"Oura","sequence":"first","affiliation":[{"name":"Institute for Animal Health, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK"}]},{"given":"M. S.","family":"Denyer","sequence":"additional","affiliation":[{"name":"Institute for Animal Health, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK"}]},{"given":"H.","family":"Takamatsu","sequence":"additional","affiliation":[{"name":"Institute for Animal Health, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK"}]},{"given":"R. M. E.","family":"Parkhouse","sequence":"additional","affiliation":[{"name":"Institute for Animal Health, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.1099\/vir.0.80058-0","article-title":"Characterization of pathogenic and non-pathogenic African swine fever virus isolates from Ornithodoros erraticus inhabiting pig premises in Portugal","volume":"85","author":"Boinas","year":"2004","journal-title":"J Gen Virol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1007\/BF01241270","article-title":"Studies to determine neutralizing antibody in sera from animals recovered from African swine fever and laboratory animals inoculated with African virus with adjuvants","volume":"20","author":"De Boer","year":"1967","journal-title":"Arch Gesamte Virusforsch"},{"key":"R3","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1006\/viro.1998.9068","article-title":"The African swine fever virus proteins p54 and p30 are involved in two distinct steps of virus attachment and both contribute to the antibody-mediated protective immune response","volume":"243","author":"Gomez-Puertas","year":"1998","journal-title":"Virology"},{"key":"R4","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S0022-1759(00)00222-2","article-title":"The cellular immune recognition of proteins expressed by an African swine fever virus random genomic library","volume":"242","author":"Jenson","year":"2000","journal-title":"J Immunol Methods"},{"key":"R5","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1099\/0022-1317-82-3-513","article-title":"The non-haemadsorbing African swine fever virus isolate ASFV\/NH\/P68 provides a model for defining the protective anti-virus immune response","volume":"82","author":"Leitao","year":"2001","journal-title":"J Gen Virol"},{"key":"R6","first-page":"450","article-title":"Serologic and immunologic studies with African swine fever virus","volume":"24","author":"Malmquist","year":"1963","journal-title":"Am J Vet Res"},{"key":"R7","first-page":"104","article-title":"Hemadsorption and cytopathic effect produced by African swine fever virus in swine bone marrow and buffy coat cultures","volume":"21","author":"Malmquist","year":"1960","journal-title":"Am J Vet Res"},{"key":"R8","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/BF01316896","article-title":"African swine fever virus specific porcine cytotoxic T cell activity","volume":"129","author":"Martins","year":"1993","journal-title":"Arch Virol"},{"key":"R9","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.virol.2003.11.011","article-title":"Neutralizing antibodies to African swine fever virus proteins p30, p54, and p72 are not sufficient for antibody-mediated protection","volume":"319","author":"Neilan","year":"2004","journal-title":"Virology"},{"key":"R10","first-page":"137","article-title":"Office International des Epizooties","volume-title":"In OIE Manual","year":"1996"},{"key":"R11","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1006\/viro.1994.1040","article-title":"Passively transferred African swine fever virus antibodies protect swine against lethal infection","volume":"198","author":"Onisk","year":"1994","journal-title":"Virology"},{"key":"R12","doi-asserted-by":"crossref","first-page":"37","DOI":"10.4049\/jimmunol.134.1.37","article-title":"Murine anti-swine T4 and T8 monoclonal antibodies: distribution and effects on proliferative and cytotoxic T cells","volume":"134","author":"Pescovitz","year":"1985","journal-title":"J Immunol"},{"key":"R13","first-page":"568","article-title":"African swine fever","volume-title":"In Infectious Diseases of Livestock","volume":"vol.\u20051","author":"Plowright","year":"1994"},{"key":"R14","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0168-1702(96)01403-7","article-title":"Modulation of immune cell populations and activation markers in the pathogenesis of African swine fever virus infection","volume":"47","author":"Ramiro-Ibanez","year":"1997","journal-title":"Virus Res"},{"key":"R15","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1111\/j.1365-3083.1992.tb02854.x","article-title":"Interferon- \u03b3 production by African swine fever virus-specific lymphocytes","volume":"35","author":"Revilla","year":"1992","journal-title":"Scand J Immunol"},{"key":"R16","first-page":"206","article-title":"Immunological responses of pigs to partially attenuated African swine fever virus and their resistance to virulent homologous and heterologous viruses","volume-title":"In African Swine Fever , EUR 8466 EN, Proceedings of CEC\/FAO Research Seminar","author":"Ruiz-Gonzalvo","year":"1983"},{"key":"R17","first-page":"1858","article-title":"Neutralization of African swine fever virus by sera from African swine fever resistant pigs","volume":"47","author":"Ruiz-Gonzalvo","year":"1986","journal-title":"Am J Vet Res"},{"key":"R18","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1006\/viro.1996.0193","article-title":"Functional and immunological properties of the baculovirus-expressed hemagglutinin of African swine fever virus","volume":"218","author":"Ruiz-Gonzalvo","year":"1996","journal-title":"Virology"},{"key":"R19","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S0167-5699(96)90273-X","article-title":"Characterization of swine leukocyte differentiation antigens","volume":"17","author":"Saalm\u00fcller","year":"1996","journal-title":"Immunol Today"},{"key":"R20","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0165-2427(02)00045-4","article-title":"T-helper cells from naive to committed","volume":"87","author":"Saalm\u00fcller","year":"2002","journal-title":"Vet Immunol Immunopathol"},{"key":"R21","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1097\/00007890-197612000-00004","article-title":"Transplantation in miniature swine. I. Fixation of major the histocompatibility complex","volume":"22","author":"Sachs","year":"1976","journal-title":"Transplantation"},{"key":"R22","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/0165-2427(85)90071-6","article-title":"The role of antibody in protection against African swine fever virus","volume":"9","author":"Wardley","year":"1985","journal-title":"Vet Immunol Immunopathol"},{"key":"R23","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1046\/j.1365-2567.1996.d01-705.x","article-title":"Phenotypic classification of porcine lymphocyte subpopulations in blood and lymphoid tissues","volume":"89","author":"Yang","year":"1996","journal-title":"Immunology"},{"key":"R24","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1046\/j.1365-2567.1997.00308.x","article-title":"Differential expression of CD8 epitopes amongst porcine CD8-positive functional lymphocyte subsets","volume":"92","author":"Yang","year":"1997","journal-title":"Immunology"},{"key":"R25","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1046\/j.1365-2567.2000.00019.x","article-title":"Characterization of the porcine \u03b3\u03b4 T-cell receptor structure and cellular distribution by monoclonal antibody PPT27","volume":"99","author":"Yang","year":"2000","journal-title":"Immunology"},{"key":"R26","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1006\/viro.1993.1515","article-title":"Virulent African swine fever virus isolates are neutralised by swine immune serum and by monoclonal antibodies recognising a 72-kDa viral protein","volume":"196","author":"Zsak","year":"1993","journal-title":"Virology"}],"container-title":["Journal of General Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/vir.0.81038-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T04:11:19Z","timestamp":1683173479000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/vir.0.81038-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,9,1]]},"references-count":26,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2005,9,1]]}},"URL":"https:\/\/doi.org\/10.1099\/vir.0.81038-0","relation":{},"ISSN":["0022-1317","1465-2099"],"issn-type":[{"value":"0022-1317","type":"print"},{"value":"1465-2099","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,9,1]]}}}