{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:15:31Z","timestamp":1791155731752,"version":"4.1.0"},"reference-count":31,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>A \u201cnovel\u201d protocol is presented for easy and reliable estimation of soluble hydroxycinnamate levels in<jats:italic>Cichorium intybus<\/jats:italic>L. leaf tissue in large-scale experiments. Samples were standardized by punching 6 discs per leaf, and hydroxycinnamates were extracted by submerging the discs in 80% ethanol with 5% acetic acid for at least 48\u2009h in the darkness at 4\u00b0C. Residual dry mass of the discs was used for<jats:italic>a posteriori<\/jats:italic>correction of compound levels. Chlorophyll was eliminated by chloroform, and the aqueous phases were transferred to microplates, dried, and dissolved in 50% methanol for HPLC analysis and storage. An HPLC program of 8\u2009min was developed for the analysis of the extracts. Comparisons with extractions of liquid nitrogen powders indicated that the novel extraction method was reliable. No degradation of the major hydroxycinnamates\u2014caftaric, chlorogenic, and chicoric acids\u2014was observed, during maceration at ambient temperatures, or after storage for 1 year.<\/jats:p>","DOI":"10.1100\/2012\/142983","type":"journal-article","created":{"date-parts":[[2012,12,5]],"date-time":"2012-12-05T23:54:07Z","timestamp":1354751647000},"page":"1-8","source":"Crossref","is-referenced-by-count":25,"title":["A \u201cNovel\u201d Protocol for the Analysis of Hydroxycinnamic Acids in Leaf Tissue of Chicory (<i>Cichorium intybus<\/i>L., Asteraceae)"],"prefix":"10.1100","volume":"2012","author":[{"given":"Meriem","family":"Bahri","sequence":"first","affiliation":[{"name":"Universit\u00e9 Lille 1, Sciences et Technologies, UMR INRA 1281 \u201cStress Abiotiques et Diff\u00e9renciation des V\u00e9g\u00e9taux Cultiv\u00e9s\u201d, GIS GENOCHIC, B\u00e2timent SN2, 3\u00e8me \u00c9tage, 59655 Villeneuve d\u2019Ascq Cedex, 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