{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T04:48:49Z","timestamp":1749185329824},"reference-count":57,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>Sprouted corm sections of \u201cSouth Dade\u201d white cocoyam were potted and maintained in a greenhouse for 8 weeks. Shoot tips of 3\u20135\u2009mm comprising the apical meristem with 4\u20136 leaf primordial, and approximately 0.5\u2009mm of corm tissue at the base. These explants were treated to be used into the culture medium. A modified Gamborg\u2019s B5 mineral salts supplemented with 0.05\u2009<jats:italic>\u03bc<\/jats:italic>M 1-naphthaleneacetic acid (NAA) were used throughout the study. Thidiazuron (TDZ) solution containing 0.01% dimethyl sulfoxide (DMSO) was used. Erlenmeyer flasks and test tubes were used for growing cultures. The effect of different media substrate, thidiazuron, and the interaction between TDZ and Benzylaminopurine (BAP) on cocoyam culture were tested. Results indicated that cocoyam can be successfully micropropagated in vitro through various procedures. All concentrations tested (5\u201320\u2009<jats:italic>\u03bc<\/jats:italic>M BAP and 1\u20134\u2009<jats:italic>\u03bc<\/jats:italic>M TDZ) produced more axillary shoots per shoot tip than the control without cytokinins. Greater proliferation rates were obtained through the use of 20\u2009<jats:italic>\u03bc<\/jats:italic>M BAP and 2\u2009<jats:italic>\u03bc<\/jats:italic>M TDZ, respectively, 12 weeks from initiation. Shoots produced with BAP were larger and more normal in appearance than those produced with TDZ, which were small, compressed, and stunted. The use of stationary liquid media is recommended for economic reasons.<\/jats:p>","DOI":"10.1100\/2012\/346595","type":"journal-article","created":{"date-parts":[[2012,4,30]],"date-time":"2012-04-30T22:02:32Z","timestamp":1335823352000},"page":"1-10","source":"Crossref","is-referenced-by-count":8,"title":["An Efficient In Vitro Propagation Protocol of Cocoyam [<i>Xanthosoma sagittifolium<\/i>(L) Schott]"],"prefix":"10.1100","volume":"2012","author":[{"given":"Anne E.","family":"Sama","sequence":"first","affiliation":[{"name":"Department of Horticulture & Landscape Architecture, Fort Collins, CO 80523-1173, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harrison G.","family":"Hughes","sequence":"additional","affiliation":[{"name":"Department of Horticulture & Landscape Architecture, Fort Collins, CO 80523-1173, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed 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