{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T11:58:31Z","timestamp":1774353511910,"version":"3.50.1"},"reference-count":55,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>The aim of this study was to detect the virulence factors, serogroups, and antibiotic resistance properties of Shiga toxin-producing<jats:italic>Escherichia coli<\/jats:italic>, by using 268 bovine mastitic milk samples which were diagnosed using California Mastitis Test. After<jats:italic>E. coli<\/jats:italic>identification, PCR assays were developed for detection of different virulence genes, serogroups, and antibiotic resistance genes of<jats:italic>Escherichia coli<\/jats:italic>. The antibiotic resistance pattern was studied using disk diffusion method. Out of 268 samples, 73 (27.23%) were positive for<jats:italic>Escherichia coli<\/jats:italic>, and, out of 73 positive samples, 15 (20.54%) were O26 and 11 (15.06%) were O157 so they were the highest while O111 was not detected in any sample so it was the lowest serogroup. Out of 73 STEC strains, 11 (15.06%) and 36 (49.31%) were EHEC and AEEC, respectively. All of the EHEC strains had<jats:italic>stx1<\/jats:italic>,<jats:italic>eaeA<\/jats:italic>, and<jats:italic>ehly<\/jats:italic>, virulence genes, while in AEEC strains<jats:italic>stx1<\/jats:italic>had the highest prevalence (77.77%), followed by<jats:italic>eaeA<\/jats:italic>(55.55%). Totally,<jats:italic>aadA1<\/jats:italic>(65.95%) had the highest while<jats:italic>blaSHV<\/jats:italic>(6.38%) had the lowest prevalence of antibiotic resistance genes. The disk diffusion method showed that the STEC strains had the highest resistance to penicillin (100%), followed by tetracycline (57.44%), while resistance to cephalothin (6.38%) was the lowest.<\/jats:p>","DOI":"10.1100\/2012\/618709","type":"journal-article","created":{"date-parts":[[2012,11,19]],"date-time":"2012-11-19T21:02:01Z","timestamp":1353358921000},"page":"1-9","source":"Crossref","is-referenced-by-count":40,"title":["Shiga Toxin-Producing<i>Escherichia coli<\/i>Isolated from Bovine Mastitic Milk: Serogroups, Virulence Factors, and Antibiotic Resistance Properties"],"prefix":"10.1100","volume":"2012","author":[{"given":"Hassan","family":"Momtaz","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, P.O. Box 166, Shahrekord, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farhad","family":"Safarpoor Dehkordi","sequence":"additional","affiliation":[{"name":"Young Researchers Club, Islamic Azad University, Shahrekord Branch, P.O. Box 166, Shahrekord, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taghi","family":"Taktaz","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, P.O. Box 166, Shahrekord, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amir","family":"Rezvani","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, P.O. Box 166, Shahrekord, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sajad","family":"Yarali","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, P.O. Box 166, Shahrekord, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"98","reference":[{"key":"1","first-page":"614","volume":"7","year":"2011","journal-title":"Journal of American Science"},{"issue":"2","key":"2","doi-asserted-by":"crossref","first-page":"264","DOI":"10.3168\/jds.S0022-0302(00)74873-9","volume":"83","year":"2000","journal-title":"Journal of Dairy Science"},{"issue":"4","key":"3","doi-asserted-by":"crossref","first-page":"567","DOI":"10.2460\/javma.2001.218.567","volume":"218","year":"2001","journal-title":"Journal of the American Veterinary Medical Association"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2005.01.007"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2005.06.009"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2009.06.002"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2010.06.002"},{"issue":"5","key":"9","first-page":"361","volume":"31","year":"2007","journal-title":"Turkish Journal of Veterinary and Animal Sciences"},{"key":"10","first-page":"15","volume":"57","year":"2004","journal-title":"Revue d'Elevage et de Medecine Veterinaire des pays Tropicaux"},{"issue":"9","key":"11","doi-asserted-by":"crossref","first-page":"3408","DOI":"10.3168\/jds.S0022-0302(06)72377-3","volume":"89","year":"2006","journal-title":"Journal of Dairy Science"},{"issue":"3","key":"12","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1128\/CMR.11.3.450","volume":"11","year":"1998","journal-title":"Clinical Microbiology Reviews"},{"issue":"12","key":"13","doi-asserted-by":"crossref","first-page":"2980","DOI":"10.1128\/JCM.34.12.2980-2984.1996","volume":"34","year":"1996","journal-title":"Journal of Clinical Microbiology"},{"issue":"3-4","key":"14","first-page":"203","volume":"44","year":"2003","journal-title":"Acta Veterinaria Scandinavica"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.04.040"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.07.011"},{"issue":"6","key":"17","doi-asserted-by":"crossref","first-page":"1795","DOI":"10.1128\/JCM.36.6.1795-1797.1998","volume":"36","year":"1998","journal-title":"Journal of Clinical Microbiology"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.7.2555-2565.2002"},{"key":"32","year":"2006"},{"issue":"7","key":"19","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1128\/JCM.30.7.1801-1806.1992","volume":"30","year":"1992","journal-title":"Journal of Clinical Microbiology"},{"issue":"1","key":"20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0950268800058064","volume":"115","year":"1995","journal-title":"Epidemiology and Infection"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-010-1036-6"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2007.06.001"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.8.4919-4924.2005"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2002.01743.x"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.3.1830-1832.2004"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.3379-3383.2003"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)00980-3"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh070"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268804003048"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.1.71-76.2005"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2008.03.029"},{"key":"33","first-page":"1","volume":"130","year":"2007","journal-title":"EFSA Journal"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268805004486"},{"issue":"2","key":"35","doi-asserted-by":"crossref","first-page":"450","DOI":"10.3168\/jds.S0022-0302(05)72706-5","volume":"88","year":"2005","journal-title":"Journal of Dairy Science"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2008-1055"},{"issue":"4","key":"37","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1051\/vetres:2000128","volume":"31","year":"2000","journal-title":"Veterinary Research"},{"issue":"1","key":"38","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1080\/01652176.2003.9695142","volume":"25","year":"2003","journal-title":"Veterinary Quarterly"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2010.0257"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.2004.00772.x"},{"issue":"28","key":"41","first-page":"4356","volume":"9","year":"2010","journal-title":"African Journal of Biotechnology"},{"key":"42","first-page":"122","volume":"13","year":"2010","journal-title":"Bulgarian Journal of Veterinary Medicine"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-54-2"},{"issue":"12","key":"44","doi-asserted-by":"crossref","first-page":"3927","DOI":"10.3168\/jds.S0022-0302(03)74001-6","volume":"86","year":"2003","journal-title":"Journal of Dairy Science"},{"key":"45","first-page":"177","volume":"67","year":"2000","journal-title":"\u00c1rea de Zoonoses e Sa\u00fade P\u00fablica"},{"issue":"5","key":"46","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.3168\/jds.S0022-0302(02)74172-6","volume":"85","year":"2002","journal-title":"Journal of Dairy Science"},{"issue":"11","key":"47","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.2460\/javma.2003.222.1582","volume":"222","year":"2003","journal-title":"Journal of the American Veterinary Medical Association"},{"issue":"3","key":"48","doi-asserted-by":"crossref","first-page":"989","DOI":"10.3168\/jds.S0022-0302(06)72164-6","volume":"89","year":"2006","journal-title":"Journal of Dairy Science"},{"key":"49","first-page":"361","volume":"17","year":"2012","journal-title":"Annals of the Romanian Society for Cell Biology"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-736X2009000500001"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.12.5834-5842.2002"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2005.03.006"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2004.02384.x"},{"issue":"22","key":"54","first-page":"3706","volume":"5","year":"2011","journal-title":"African Journal of Microbiology Research"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.2.576-581.2002"},{"key":"56","first-page":"777","volume":"1","year":"2012","journal-title":"Journal of Microbiology, Biotechnology and Food Sciences"}],"container-title":["The Scientific World Journal"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2012\/618709.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2012\/618709.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2012\/618709.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T09:09:21Z","timestamp":1594890561000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/tswj\/2012\/618709\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":55,"alternative-id":["618709","618709"],"URL":"https:\/\/doi.org\/10.1100\/2012\/618709","relation":{},"ISSN":["1537-744X"],"issn-type":[{"value":"1537-744X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012]]}}}