{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T04:32:07Z","timestamp":1769315527182,"version":"3.49.0"},"reference-count":9,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>Our investigations focused on spindle dynamics\/displacement in frozen-thawed human oocytes. In each oocyte, prior to freezing and after thawing and culturing, the presence\/location of the spindle was determined with the Polscope technique. A total of 259 oocytes have been thawed with a survival rate of 81.1%. From the 210 survived oocytes, 165 were fertilized (78.6%) and 89.1% of them cleaved. A total of 143 embryos were transferred into 63 patients resulting in 11 clinical pregnancies (17.5%), 7 of which resulted in live birth of 8 babies (1 twin pregnancy). We were able to detect the spindle in 221 of 259 oocytes (85.3%). After thawing and culturing the oocytes, we were able to visualize the spindle in 177 of 210 oocytes (84.3%). In 83 of these 177 oocytes, the spindle was observed to be in the same location as it was before cryopreservation (46.9%). However, in 94 of these 177 oocytes (53.1%), the spindle reformed in a different position\/location relative to the polar body. Our results show that after thawing and culture in half of the spindle-positive oocytes the spindle was detected in a new location, indicating that the spindle and the polar body move relative to each other.<\/jats:p>","DOI":"10.1100\/2012\/785421","type":"journal-article","created":{"date-parts":[[2012,5,2]],"date-time":"2012-05-02T22:05:04Z","timestamp":1335996304000},"page":"1-4","source":"Crossref","is-referenced-by-count":7,"title":["Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication"],"prefix":"10.1100","volume":"2012","author":[{"given":"J\u00e1nos","family":"Konc","sequence":"first","affiliation":[{"name":"Infertility and IVF Center of Buda, Szent Janos Hospital, Budapest 1125, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katalin","family":"Kanyo","sequence":"additional","affiliation":[{"name":"Infertility and IVF Center of Buda, Szent Janos Hospital, Budapest 1125, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"Kriston","sequence":"additional","affiliation":[{"name":"Infertility and IVF Center of Buda, Szent Janos Hospital, Budapest 1125, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f3zsef","family":"Zeke","sequence":"additional","affiliation":[{"name":"Infertility and IVF Center of Buda, Szent Janos Hospital, Budapest 1125, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e1ndor","family":"Cseh","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science, Szent Istv\u00e1n University, Istv\u00e1n u. 2, Budapest 1078, Hungary"},{"name":"Subsidized Research Unit, Hungarian Academy of Sciences, 1525 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"98","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh101"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(00)01800-8"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.10.006"},{"issue":"3","key":"4","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/S1472-6483(10)60218-0","volume":"17","year":"2008","journal-title":"Reproductive BioMedicine Online"},{"key":"5","first-page":"57","volume":"14","year":"2006","journal-title":"Reproductive BioMedicine Online"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmm028"},{"key":"7","first-page":"114","volume-title":"Cryopreservation of human oocytes: an overview","year":"2010"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60071-5"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2008.03.013"}],"container-title":["The Scientific World Journal"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2012\/785421.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2012\/785421.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2012\/785421.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,20]],"date-time":"2017-06-20T18:16:03Z","timestamp":1497982563000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/tswj\/2012\/785421\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":9,"alternative-id":["785421","785421"],"URL":"https:\/\/doi.org\/10.1100\/2012\/785421","relation":{},"ISSN":["1537-744X"],"issn-type":[{"value":"1537-744X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012]]}}}