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We describe here an experimental approach that can accurately identify the proteins present in the WSM of shell mollusk nacre. Four proteins (three gigasin-2 isoforms and a cystatin A2) were for the first time identified in WSM of<jats:italic>Crassostrea gigas<\/jats:italic>nacre using 2DE and LC-MS\/MS for protein identification. These proteins are thought to be involved in bone remodeling processes and could be responsible for the biocompatibility shown between bone and nacre grafts. These results represent a contribution to the study of shell biomineralization process and opens new perspectives for the development of new nacre biomaterials for orthopedic applications.<\/jats:p>","DOI":"10.1100\/2012\/765909","type":"journal-article","created":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T22:03:19Z","timestamp":1335909799000},"page":"1-9","source":"Crossref","is-referenced-by-count":20,"title":["Identification of Proteins with Potential Osteogenic Activity Present in the Water-Soluble Matrix Proteins from<i>Crassostrea gigas<\/i>Nacre Using a Proteomic Approach"],"prefix":"10.1100","volume":"2012","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0622-2934","authenticated-orcid":true,"given":"Daniel V.","family":"Oliveira","sequence":"first","affiliation":[{"name":"Center of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9434-8686","authenticated-orcid":true,"given":"Tom\u00e9 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