{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T15:59:16Z","timestamp":1768492756662,"version":"3.49.0"},"posted":{"date-parts":[[2020,9,13]]},"group-title":"Immunology","reference-count":26,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2020,9,13]],"date-time":"2020-09-13T00:00:00Z","timestamp":1599955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2020,9,13]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently causing a global pandemic. The antigen specificity and kinetics of the antibody response mounted against this novel virus are not understood in detail. Here, we report that subjects with a more severe SARS-CoV-2 infection exhibit a larger antibody response against the spike and nucleocapsid protein and epitope spreading to subdominant viral antigens, such as open reading frame 8 and non-structural proteins. Subjects with a greater antibody response mounted a larger memory B cell response against the spike, but not the nucleocapsid protein. Additionally, we revealed that antibodies against the spike are still capable of binding the D614G spike mutant and cross-react with the SARS-CoV-1 receptor binding domain. Together, this study reveals that subjects with a more severe SARS-CoV-2 infection exhibit a greater overall antibody response to the spike and nucleocapsid protein and a larger memory B cell response against the spike.<\/jats:p>","DOI":"10.1101\/2020.09.12.294066","type":"posted-content","created":{"date-parts":[[2020,9,13]],"date-time":"2020-09-13T23:00:11Z","timestamp":1600038011000},"source":"Crossref","is-referenced-by-count":8,"title":["SARS-CoV-2 infection severity is linked to superior humoral immunity against the spike"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7566-3536","authenticated-orcid":false,"given":"Jenna 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