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Widespread detection of variant strains will be critical to inform policy decisions to mitigate further spread, and post-pandemic multiplexed screening of respiratory viruses will be necessary to properly manage patients presenting with similar respiratory symptoms. In this work, we have developed a portable, magnetofluidic cartridge platform for automated PCR testing in &lt;30 min. Cartridges were designed for multiplexed detection of SARS-CoV-2 with either distinctive variant mutations or with Influenza A and B. The platform demonstrated a limit of detection down to 2 copies\/\u00b5L SARS-CoV-2 RNA with successful identification of B.1.1.7 and B.1.351 variants. The multiplexed SARS-CoV-2\/Flu assay was validated using archived clinical nasopharyngeal swab eluates (\n                  <jats:italic>n<\/jats:italic>\n                  = 116) with an overall sensitivity\/specificity of 98.1%\/95.2%, 85.7%\/100%, 100%\/98.2%, respectively, for SARS-CoV-2, Influenza A, and Influenza B. Further testing with saliva (\n                  <jats:italic>n<\/jats:italic>\n                  = 14) demonstrated successful detection of all SARS-CoV-2 positive samples with no false-positives.\n                <\/jats:p>","DOI":"10.1101\/2021.05.10.21256995","type":"posted-content","created":{"date-parts":[[2021,5,11]],"date-time":"2021-05-11T23:16:03Z","timestamp":1620774963000},"source":"Crossref","is-referenced-by-count":3,"title":["Magnetofluidic platform for rapid multiplexed screening of SARS-CoV-2 variants and respiratory pathogens"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3997-9669","authenticated-orcid":false,"given":"Alexander Y.","family":"Trick","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan-En","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liben","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei-Wei","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander C.","family":"Hasnain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heba H.","family":"Mostafa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen C.","family":"Carroll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tza-Huei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2021051309300454000_2021.05.10.21256995v1.1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc2009316 [published Online First: 2020\/04\/14]"},{"key":"2021051309300454000_2021.05.10.21256995v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30457-6"},{"key":"2021051309300454000_2021.05.10.21256995v1.3","first-page":"0","article-title":"Weekly COVID-19 testing with household quarantine and contact tracing is feasible and would probably end the epidemic: Weekly Covid-19 testing","volume":"7","year":"2020","journal-title":"R. 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