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Many are also difficult to implement or inaccessible without specialized liquid handlers, further limiting their utility. We present Single-cell TOtal RNA-seq Miniaturized (STORM-seq), a random- hexamer primed, ribo-reduced single-cell total RNA sequencing (sc-total-RNA-seq) protocol using standard laboratory equipment. Adapted as a kit, STORM-seq constructs sequence-ready libraries in one working day, producing the highest complexity scRNA- seq libraries to-date, robustly measuring transcript isoforms and clinically relevant gene fusions in single cells. STORM-seq faithfully reconstructs expression profiles of locus- level transposable elements (TEs), and provides high-resolution profiling of transient, low- abundance enhancer RNAs (eRNAs), offering a powerful tool to dissect single-cell gene regulatory networks in unprecedented detail. Applied to human fallopian tube epithelium, the improved transcriptional resolution reveals a putative progenitor-like population and intermediate cell states, shaped by TEs and non-coding RNAs.<\/jats:p>","DOI":"10.1101\/2022.03.14.484332","type":"posted-content","created":{"date-parts":[[2022,3,14]],"date-time":"2022-03-14T23:00:27Z","timestamp":1647298827000},"source":"Crossref","is-referenced-by-count":17,"title":["Efficient profiling of total RNA in single cells with STORM-seq"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1482-1032","authenticated-orcid":false,"given":"Benjamin K.","family":"Johnson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary F.","family":"Majewski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9654-4448","authenticated-orcid":false,"given":"H. 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