{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T17:27:38Z","timestamp":1768843658620,"version":"3.49.0"},"posted":{"date-parts":[[2022,12,27]]},"group-title":"Immunology","reference-count":69,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2022,12,27]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>Prime-boost regimens for COVID-19 vaccines elicit poor antibody responses against Omicron-based variants and employ frequent boosters to maintain antibody levels. We present a natural infection-mimicking technology that combines features of mRNA- and protein nanoparticle-based vaccines through encoding self-assembling enveloped virus-like particles (eVLPs). eVLP assembly is achieved by inserting an ESCRT- and ALIX-binding region (EABR) into the SARS-CoV-2 spike cytoplasmic tail, which recruits ESCRT proteins to induce eVLP budding from cells. Purified spike-EABR eVLPs presented densely-arrayed spikes and elicited potent antibody responses in mice. Two immunizations with mRNA-LNP encoding spike-EABR elicited potent CD8+ T-cell responses and superior neutralizing antibody responses against original and variant SARS-CoV-2 compared to conventional spike-encoding mRNA-LNP and purified spike-EABR eVLPs, improving neutralizing titers &gt;10-fold against Omicron-based variants for three months post-boost. Thus, EABR technology enhances potency and breadth of vaccine-induced responses through antigen presentation on cell surfaces and eVLPs, enabling longer-lasting protection against SARS-CoV-2 and other viruses.<\/jats:p>","DOI":"10.1101\/2022.12.26.521940","type":"posted-content","created":{"date-parts":[[2022,12,27]],"date-time":"2022-12-27T18:20:11Z","timestamp":1672165211000},"source":"Crossref","is-referenced-by-count":1,"title":["ESCRT recruitment to mRNA-encoded SARS-CoV-2 spike induces virus-like particles and enhanced antibody responses"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4923-9568","authenticated-orcid":false,"given":"Magnus A. 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