{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T19:30:25Z","timestamp":1768505425720,"version":"3.49.0"},"posted":{"date-parts":[[2023,1,28]]},"group-title":"Microbiology","reference-count":163,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,7,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Crohn\u2019s Disease (CD) is a presentation of Inflammatory Bowel Disease (IBD) that manifests in childhood and adolescence, and involves chronic and severe enterocolitis, immune and gut microbiome dysregulation, and other complications. Diet and gut-microbiota-produced metabolites are sources of anti-inflammatories which could ameliorate symptoms. However, questions remain on how IBD influences biogeographic patterns of microbial location and function in the gut, how early life transitional gut communities are affected by IBD and diet interventions, and how disruption to biogeography alters disease mediation by diet components or microbial metabolites. Many studies on diet and IBD use a chemically induced ulcerative colitis model, despite the availability of an immune-modulated CD model. Interleukin-10-knockout (IL-10-KO) mice on a C57BL\/6 background, beginning at age 4 or 7 weeks, were fed a control diet or one containing 10% (w\/w) raw broccoli sprouts, which was high in the sprout-sourced anti-inflammatory sulforaphane. Diets began 7 days prior to, and for 2 weeks after inoculation with\n                  <jats:italic>Helicobacter hepaticus,<\/jats:italic>\n                  which triggers Crohn\u2019s-like symptoms in these immune-impaired mice. The broccoli sprout diet increased sulforaphane in plasma; decreased weight stagnation, fecal blood, and diarrhea associated; and increased microbiota richness in the gut, especially in younger mice. Sprout diets resulted in some anatomically specific bacteria in younger mice, and reduced the prevalence and abundance of pathobiont bacteria which trigger inflammation in the IL-10-KO mouse, for example;\n                  <jats:italic>Escherichia coli<\/jats:italic>\n                  and\n                  <jats:italic>Helicobacter<\/jats:italic>\n                  . Overall, the IL-10-KO mouse model is responsive to a raw broccoli sprout diet and represents an opportunity for more diet-host-microbiome research.\n                <\/jats:p>\n                <jats:sec id=\"s2\">\n                  <jats:title>Importance<\/jats:title>\n                  <jats:p>To our knowledge, IL-10-KO mice have not previously been used to investigate the interactions of host, microbiota, and broccoli, broccoli sprouts, or broccoli bioactives in resolving symptoms of CD. We showed that a diet containing 10% raw broccoli sprouts increased the plasma concentration of the anti-inflammatory compound sulforaphane, and protected mice to varying degrees against negative disease symptoms, including weight loss or stagnation, fecal blood, and diarrhea. Younger mice responded more strongly to the diet, further reducing symptoms, as well as increased gut bacterial community richness, increased bacterial community similarity to each other, and more location-specific communities than older mice on the diet intervention. Crohn\u2019s Disease disrupts the lives of patients, and requires people to alter dietary and lifestyle habits to manage symptoms. The current medical treatment is extremely expensive, and a dietary intervention represents an affordable, accessible, and simple strategy to reduce the burden of symptoms.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2023.01.27.525953","type":"posted-content","created":{"date-parts":[[2023,1,28]],"date-time":"2023-01-28T21:10:11Z","timestamp":1674940211000},"source":"Crossref","is-referenced-by-count":1,"title":["Early life exposure to broccoli sprouts confers stronger protection against enterocolitis development in an immunological mouse model of inflammatory bowel disease"],"prefix":"10.64898","author":[{"given":"Lola","family":"Holcomb","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johanna 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