{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T19:06:16Z","timestamp":1768503976126,"version":"3.49.0"},"posted":{"date-parts":[[2023,2,22]]},"group-title":"Molecular Biology","reference-count":40,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,12,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Mutations in\n                  <jats:italic>CHCHD10<\/jats:italic>\n                  , a mitochondrial protein with undefined functions, are associated with autosomal dominant mitochondrial diseases.\n                  <jats:italic>Chchd10<\/jats:italic>\n                  knock-in mice harboring a heterozygous S55L mutation (equivalent to human pathogenic S59L) develop a fatal mitochondrial cardiomyopathy caused by CHCHD10 aggregation and proteotoxic mitochondrial integrated stress response (mtISR). In mutant hearts, mtISR is accompanied by a metabolic rewiring characterized by increased reliance on glycolysis rather than fatty acid oxidation. To counteract this metabolic rewiring, heterozygous S55L mice were subjected to chronic high fat diet (HFD) to decrease insulin sensitivity and glucose uptake and enhance fatty acid utilization in the heart. HFD ameliorated the ventricular dysfunction of mutant hearts and significantly extended the survival of mutant female mice affected by severe pregnancy-induced cardiomyopathy. Gene expression profiles confirmed that HFD increased fatty acid utilization and ameliorated cardiomyopathy markers. Importantly, HFD also decreased accumulation of aggregated CHCHD10 in the S55L heart, suggesting activation of quality control mechanisms. Overall, our findings indicate that metabolic therapy can be effective in mitochondrial cardiomyopathies associated with proteotoxic stress.\n                <\/jats:p>","DOI":"10.1101\/2023.02.22.529577","type":"posted-content","created":{"date-parts":[[2023,2,22]],"date-time":"2023-02-22T20:20:18Z","timestamp":1677097218000},"source":"Crossref","is-referenced-by-count":1,"title":["High fat diet ameliorates mitochondrial cardiomyopathy in CHCHD10 mutant mice"],"prefix":"10.64898","author":[{"given":"Nneka","family":"Southwell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Onorina","family":"Manzo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dazhi","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole M.","family":"Sayles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jalia","family":"Dash","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keigo","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilena","family":"D\u2019Aurelio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annarita","family":"Di Lorenzo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanni","family":"Manfredi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hibiki","family":"Kawamata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023123002000926000_2023.02.22.529577v2.1","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1016\/j.cmet.2022.08.017","article-title":"OMA1-mediated integrated stress response protects against ferroptosis in mitochondrial cardiomyopathy","volume":"34","year":"2022","journal-title":"Cell Metab"},{"key":"2023123002000926000_2023.02.22.529577v2.2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-019-01989-y"},{"key":"2023123002000926000_2023.02.22.529577v2.3","doi-asserted-by":"publisher","DOI":"10.1212\/NXG.0000000000000003"},{"key":"2023123002000926000_2023.02.22.529577v2.4","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awu138"},{"key":"2023123002000926000_2023.02.22.529577v2.5","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddx397"},{"key":"2023123002000926000_2023.02.22.529577v2.6","doi-asserted-by":"crossref","first-page":"1862","DOI":"10.2337\/db21-0999","article-title":"CHCHD10 Modulates Thermogenesis of Adipocytes by Regulating Lipolysis","volume":"71","year":"2022","journal-title":"Diabetes"},{"key":"2023123002000926000_2023.02.22.529577v2.7","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1038\/s41586-020-2076-4","article-title":"A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol","volume":"579","year":"2020","journal-title":"Nature"},{"key":"2023123002000926000_2023.02.22.529577v2.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2020.01.006"},{"key":"2023123002000926000_2023.02.22.529577v2.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2018.07.027"},{"key":"2023123002000926000_2023.02.22.529577v2.10","doi-asserted-by":"crossref","first-page":"3415","DOI":"10.1093\/brain\/awac197","article-title":"CHCHD10 and SLP2 control the stability of the PHB complex: a key factor for motor neuron viability","volume":"145","year":"2022","journal-title":"Brain"},{"key":"2023123002000926000_2023.02.22.529577v2.11","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-019-01988-z"},{"key":"2023123002000926000_2023.02.22.529577v2.12","doi-asserted-by":"crossref","unstructured":"Gostimskaya I , Galkin A (2010) Preparation of highly coupled rat heart mitochondria. 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