{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T23:02:48Z","timestamp":1778108568225,"version":"3.51.4"},"posted":{"date-parts":[[2023,2,24]]},"group-title":"Immunology","reference-count":74,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,2,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Defending against future pandemics may require vaccine platforms that protect across a range of related pathogens. The presentation of multiple receptor-binding domains (RBDs) from evolutionarily-related viruses on a nanoparticle scaffold elicits a strong antibody response to conserved regions. Here we produce quartets of tandemly-linked RBDs from SARS-like betacoronaviruses coupled to the mi3 nanocage through a SpyTag\/SpyCatcher spontaneous reaction. These Quartet Nanocages induce a high level of neutralizing antibodies against several different coronaviruses, including against viruses not represented on the vaccine. In animals primed with SARS-CoV-2 Spike, boost immunizations with Quartet Nanocages increased the strength and breadth of an otherwise narrow immune response. Quartet Nanocages are a strategy with potential to confer heterotypic protection against emergent zoonotic coronavirus pathogens and facilitate proactive pandemic protection.<\/jats:p>\n                <jats:sec>\n                  <jats:title>One Sentence Summary<\/jats:title>\n                  <jats:p>A vaccine candidate with polyprotein antigens displayed on nanocages induces neutralizing antibodies to multiple SARS-like coronaviruses.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2023.02.24.529520","type":"posted-content","created":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T17:40:11Z","timestamp":1677260411000},"source":"Crossref","is-referenced-by-count":10,"title":["Multiviral Quartet Nanocages Elicit Broad Anti-Coronavirus Responses for Proactive Vaccinology"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5698-9538","authenticated-orcid":false,"given":"Rory 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