{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:38:11Z","timestamp":1790509091182,"version":"4.1.0"},"posted":{"date-parts":[[2023,3,6]]},"group-title":"Neuroscience","reference-count":77,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,3,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Human cytomegalovirus (HCMV) is a highly prevalent viral pathogen that typically presents asymptomatically in healthy individuals despite lifelong latency. However, in 10-15% of congenital cases, this beta-herpesvirus demonstrates direct effects on the central nervous system, including microcephaly, cognitive\/learning delays, and hearing deficits. HCMV has been widely shown to infect neural progenitor cells, but the permissiveness of fully differentiated neurons to HCMV is controversial and chronically understudied, despite potential associations between HCMV infection with neurodegenerative conditions. Using a model system representative of the human forebrain, we demonstrate that induced pluripotent stem cell (iPSC)-derived, excitatory glutamatergic and inhibitory GABAergic neurons are fully permissive to HCMV, demonstrating complete viral replication, competent virion production, and spread within the culture. Interestingly, while cell proliferation was not induced in these post-mitotic neurons, HCMV did increase expression of proliferative markers Ki67 and PCNA suggesting alterations in cell cycle machinery. These finding are consistent with previous HCMV-mediated changes in various cell types and implicate the virus\u2019 ability to alter proliferative pathways to promote virion production. HCMV also induces significant structural changes in forebrain neurons, such as the formation of syncytia and retraction of neurites. Finally, we demonstrate that HCMV disrupts calcium signaling and decreases neurotransmission, with action potential generation effectively silenced after 15 days post infection. Taken together, our data highlight the potential for forebrain neurons to be permissive to HCMV infection in the CNS, which could have significant implications on overall brain health and function.<\/jats:p>","DOI":"10.1101\/2023.03.03.531045","type":"posted-content","created":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T17:00:13Z","timestamp":1678294813000},"source":"Crossref","is-referenced-by-count":3,"title":["Human Cytomegalovirus Induces Significant Structural and Functional Changes in Terminally Differentiated Human Cortical Neurons"],"prefix":"10.64898","author":[{"given":"Jacob W.","family":"Adelman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzette","family":"Rosas-Rogers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan L.","family":"Schumacher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekah L.","family":"Mokry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott S.","family":"Terhune","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allison D.","family":"Ebert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2023030900201420000_2023.03.03.531045v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-021-00582-z"},{"key":"2023030900201420000_2023.03.03.531045v1.2","doi-asserted-by":"publisher","DOI":"10.1159\/000322141"},{"key":"2023030900201420000_2023.03.03.531045v1.3","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2034"},{"key":"2023030900201420000_2023.03.03.531045v1.4","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1186\/s13052-022-01387-3","article-title":"Early neurodevelopmental outcomes in children with asymptomatic congenital CMV infection","volume":"48","year":"2022","journal-title":"Italian Journal of Pediatrics"},{"key":"2023030900201420000_2023.03.03.531045v1.5","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-4-22"},{"key":"2023030900201420000_2023.03.03.531045v1.6","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit018"},{"key":"2023030900201420000_2023.03.03.531045v1.7","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02161-09"},{"key":"2023030900201420000_2023.03.03.531045v1.8","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.535"},{"key":"2023030900201420000_2023.03.03.531045v1.9","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.544"},{"key":"2023030900201420000_2023.03.03.531045v1.10","doi-asserted-by":"publisher","DOI":"10.1001\/archpedi.1982.03970460032007"},{"key":"2023030900201420000_2023.03.03.531045v1.11","doi-asserted-by":"publisher","DOI":"10.1097\/CM9.0000000000000404"},{"key":"2023030900201420000_2023.03.03.531045v1.12","doi-asserted-by":"crossref","unstructured":"Turriziani Colonna A , Buonsenso D , Pata D , Salerno G , Chieffo DPR , Romeo DM , Faccia V , Conti G , Molle F , Baldascino A , De Waure C , Acampora A , Luciano R , Santangelo R , Valentini P. 2020. 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