{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T19:43:28Z","timestamp":1768506208637,"version":"3.49.0"},"posted":{"date-parts":[[2023,3,6]]},"group-title":"Neuroscience","reference-count":60,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,3,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Axon initial segment (AIS) cell surface proteins mediate key biological processes in neurons including action potential initiation and axo-axonic synapse formation. However, few AIS cell surface proteins have been identified. Here, we used antibody-directed proximity biotinylation to define the cell surface proteins in close proximity to the AIS cell adhesion molecule Neurofascin. To determine the distributions of the identified proteins, we used CRISPR-mediated genome editing for insertion of epitope tags in the endogenous proteins. We found Contactin-1 (Cntn1) among the previously unknown AIS proteins we identified. Cntn1 is enriched at the AIS through interactions with Neurofascin and NrCAM. We further show that Cntn1 contributes to assembly of the AIS-extracellular matrix, and is required for AIS axo-axonic innervation by inhibitory basket cells in the cerebellum and inhibitory chandelier cells in the cortex.<\/jats:p>","DOI":"10.1101\/2023.03.06.531378","type":"posted-content","created":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T03:10:43Z","timestamp":1678158643000},"source":"Crossref","is-referenced-by-count":1,"title":["Antibody-directed extracellular proximity biotinylation reveals Contactin-1 regulates axo-axonic innervation of axon initial segments"],"prefix":"10.64898","author":[{"given":"Yuki","family":"Ogawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian C.","family":"Lim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanu","family":"George","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan A.","family":"Oses-Prieto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua M.","family":"Rasband","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yael","family":"Eshed-Eisenbach","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Supna","family":"Nair","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Boato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elior","family":"Peles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alma L.","family":"Burlingame","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda Van","family":"Aelst","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8184-2477","authenticated-orcid":false,"given":"Matthew N.","family":"Rasband","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023031000200976000_2023.03.06.531378v1.1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1922-17.2018"},{"key":"2023031000200976000_2023.03.06.531378v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2852"},{"key":"2023031000200976000_2023.03.06.531378v1.3","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.R120.001941"},{"key":"2023031000200976000_2023.03.06.531378v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13658-5"},{"key":"2023031000200976000_2023.03.06.531378v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.12.029"},{"key":"2023031000200976000_2023.03.06.531378v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2022.09.025"},{"key":"2023031000200976000_2023.03.06.531378v1.7","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2926-0"},{"key":"2023031000200976000_2023.03.06.531378v1.8","doi-asserted-by":"publisher","DOI":"10.1002\/cpps.27"},{"key":"2023031000200976000_2023.03.06.531378v1.9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0710346105"},{"key":"2023031000200976000_2023.03.06.531378v1.10","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4533"},{"key":"2023031000200976000_2023.03.06.531378v1.11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2203027119"},{"key":"2023031000200976000_2023.03.06.531378v1.12","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.21392"},{"key":"2023031000200976000_2023.03.06.531378v1.13","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.60619"},{"key":"2023031000200976000_2023.03.06.531378v1.14","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200610128"},{"key":"2023031000200976000_2023.03.06.531378v1.15","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200705119"},{"key":"2023031000200976000_2023.03.06.531378v1.16","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.66491"},{"key":"2023031000200976000_2023.03.06.531378v1.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2012.11.002"},{"key":"2023031000200976000_2023.03.06.531378v1.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2012.03.003"},{"key":"2023031000200976000_2023.03.06.531378v1.19","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.256180"},{"key":"2023031000200976000_2023.03.06.531378v1.20","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1698-10.2010"},{"key":"2023031000200976000_2023.03.06.531378v1.21","doi-asserted-by":"publisher","DOI":"10.1038\/nature20565"},{"key":"2023031000200976000_2023.03.06.531378v1.22","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.05.047"},{"key":"2023031000200976000_2023.03.06.531378v1.23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000665"},{"key":"2023031000200976000_2023.03.06.531378v1.24","doi-asserted-by":"crossref","first-page":"27163","DOI":"10.1016\/S0021-9258(18)46961-X","article-title":"Ankyrin binding activity shared by the neurofascin\/L1\/NrCAM family of nervous system cell adhesion molecules","volume":"269","year":"1994","journal-title":"J Biol Chem"},{"key":"2023031000200976000_2023.03.06.531378v1.25","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4302-06.2007"},{"key":"2023031000200976000_2023.03.06.531378v1.26","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.202267"},{"key":"2023031000200976000_2023.03.06.531378v1.27","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.135.5.1355"},{"key":"2023031000200976000_2023.03.06.531378v1.28","doi-asserted-by":"publisher","DOI":"10.4161\/cam.3.1.7764"},{"key":"2023031000200976000_2023.03.06.531378v1.29","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00680-7"},{"key":"2023031000200976000_2023.03.06.531378v1.30","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00296-3"},{"key":"2023031000200976000_2023.03.06.531378v1.31","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-22-08354.2000"},{"key":"2023031000200976000_2023.03.06.531378v1.32","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1313769110"},{"key":"2023031000200976000_2023.03.06.531378v1.33","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.142.4.1083"},{"key":"2023031000200976000_2023.03.06.531378v1.34","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200309147"},{"key":"2023031000200976000_2023.03.06.531378v1.35","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1346-15.2015"},{"key":"2023031000200976000_2023.03.06.531378v1.36","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(96)00120-9"},{"key":"2023031000200976000_2023.03.06.531378v1.37","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.10.004"},{"key":"2023031000200976000_2023.03.06.531378v1.38","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2021.09.033"},{"key":"2023031000200976000_2023.03.06.531378v1.39","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.73783"},{"key":"2023031000200976000_2023.03.06.531378v1.40","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2020.05.003"},{"key":"2023031000200976000_2023.03.06.531378v1.41","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.02.009"},{"key":"2023031000200976000_2023.03.06.531378v1.42","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.143.5.1295"},{"key":"2023031000200976000_2023.03.06.531378v1.43","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200806112"},{"key":"2023031000200976000_2023.03.06.531378v1.44","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201907048"},{"key":"2023031000200976000_2023.03.06.531378v1.45","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.5.978"},{"key":"2023031000200976000_2023.03.06.531378v1.46","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.119.1.203"},{"key":"2023031000200976000_2023.03.06.531378v1.47","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90312-7"},{"key":"2023031000200976000_2023.03.06.531378v1.48","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-19-07517.2001"},{"key":"2023031000200976000_2023.03.06.531378v1.49","doi-asserted-by":"publisher","DOI":"10.1002\/pd.5809"},{"key":"2023031000200976000_2023.03.06.531378v1.50","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3624"},{"key":"2023031000200976000_2023.03.06.531378v1.51","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200109026"},{"key":"2023031000200976000_2023.03.06.531378v1.52","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2011.02.021"},{"key":"2023031000200976000_2023.03.06.531378v1.53","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abf1600"},{"key":"2023031000200976000_2023.03.06.531378v1.54","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1919528117"},{"key":"2023031000200976000_2023.03.06.531378v1.55","doi-asserted-by":"publisher","DOI":"10.1126\/science.1227622"},{"key":"2023031000200976000_2023.03.06.531378v1.56","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.010728"},{"key":"2023031000200976000_2023.03.06.531378v1.57","doi-asserted-by":"publisher","DOI":"10.1021\/ac9810516"},{"key":"2023031000200976000_2023.03.06.531378v1.58","doi-asserted-by":"publisher","DOI":"10.1038\/s10038-017-0352-4"},{"key":"2023031000200976000_2023.03.06.531378v1.59","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2020.108914"},{"key":"2023031000200976000_2023.03.06.531378v1.60","doi-asserted-by":"publisher","DOI":"10.3791\/51863"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2023.03.06.531378","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T04:21:41Z","timestamp":1768450901000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2023.03.06.531378"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,6]]},"references-count":60,"URL":"https:\/\/doi.org\/10.1101\/2023.03.06.531378","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1038\/s41467-023-42273-8","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2023,3,6]]},"subtype":"preprint"}}