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Here, we demonstrate the delivery of approximately the same amount of CAR gene coding mRNA into each T cell propose an acoustic-electric microfluidic platform to manipulate cell membranes and achieve dosage control via uniform mixing, which delivers approximately the same amount of CAR genes into each T cell. We also show that CAR expression density can be titered on the surface of primary T cells under various input power conditions using the microfluidic platform.<\/jats:p>","DOI":"10.1101\/2023.03.14.532624","type":"posted-content","created":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T11:00:41Z","timestamp":1678878041000},"source":"Crossref","is-referenced-by-count":1,"title":["Titering of Chimeric Antigen Receptors on CAR T Cells enabled by a Microfluidic-based Dosage-Controlled Intracellular mRNA Delivery Platform"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9867-2045","authenticated-orcid":false,"given":"Yu-Hsi","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9499-1129","authenticated-orcid":false,"given":"Ruoyu","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8378-6616","authenticated-orcid":false,"given":"Abraham P.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023031712550853000_2023.03.14.532624v1.1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1709919"},{"key":"2023031712550853000_2023.03.14.532624v1.2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1709866"},{"key":"2023031712550853000_2023.03.14.532624v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2017.266"},{"key":"2023031712550853000_2023.03.14.532624v1.4","first-page":"578","volume":"130","year":"2017","journal-title":"Blood"},{"key":"2023031712550853000_2023.03.14.532624v1.5","doi-asserted-by":"crossref","first-page":"16011","DOI":"10.1038\/mto.2016.11","volume":"3","year":"2016","journal-title":"Molecular Therapy - Oncolytics"},{"key":"2023031712550853000_2023.03.14.532624v1.6","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.omtm.2021.03.007","volume":"21","year":"2021","journal-title":"Molecular Therapy - Methods & Clinical Development"},{"key":"2023031712550853000_2023.03.14.532624v1.7","doi-asserted-by":"crossref","first-page":"eabo0514","DOI":"10.1126\/sciadv.abo0514","volume":"8","year":"2022","journal-title":"Science Advances"},{"key":"2023031712550853000_2023.03.14.532624v1.8","doi-asserted-by":"crossref","first-page":"16015","DOI":"10.1038\/mto.2016.15","volume":"3","year":"2016","journal-title":"Molecular Therapy - Oncolytics"},{"key":"2023031712550853000_2023.03.14.532624v1.9","doi-asserted-by":"publisher","DOI":"10.1172\/JCI35700"},{"key":"2023031712550853000_2023.03.14.532624v1.10","doi-asserted-by":"crossref","first-page":"42","DOI":"10.3390\/diseases6020042","volume":"6","year":"2018","journal-title":"Diseases"},{"key":"2023031712550853000_2023.03.14.532624v1.11","doi-asserted-by":"publisher","DOI":"10.1038\/nature21405"},{"key":"2023031712550853000_2023.03.14.532624v1.12","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12137"},{"key":"2023031712550853000_2023.03.14.532624v1.13","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(01)76055-9"},{"key":"2023031712550853000_2023.03.14.532624v1.14","first-page":"e6644685","volume":"2021","year":"2021","journal-title":"Journal of Immunology Research"},{"key":"2023031712550853000_2023.03.14.532624v1.15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1809671115"},{"key":"2023031712550853000_2023.03.14.532624v1.16","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aat8131"},{"key":"2023031712550853000_2023.03.14.532624v1.17","doi-asserted-by":"crossref","first-page":"18045","DOI":"10.1038\/s41598-020-73755-0","volume":"10","year":"2020","journal-title":"Sci Rep"},{"key":"2023031712550853000_2023.03.14.532624v1.18","doi-asserted-by":"crossref","first-page":"3214","DOI":"10.1038\/s41598-019-40147-y","volume":"9","year":"2019","journal-title":"Sci Rep"},{"key":"2023031712550853000_2023.03.14.532624v1.19","doi-asserted-by":"crossref","first-page":"2102021","DOI":"10.1002\/advs.202102021","volume":"9","year":"2022","journal-title":"Advanced Science"},{"key":"2023031712550853000_2023.03.14.532624v1.20","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2017.243"},{"key":"2023031712550853000_2023.03.14.532624v1.21","doi-asserted-by":"crossref","first-page":"6514","DOI":"10.3390\/ijms21186514","volume":"21","year":"2020","journal-title":"International Journal of Molecular Sciences"},{"key":"2023031712550853000_2023.03.14.532624v1.22","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-018-0006-z"},{"key":"2023031712550853000_2023.03.14.532624v1.23","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.03.014"},{"key":"2023031712550853000_2023.03.14.532624v1.24","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.omtm.2020.01.012","volume":"16","year":"2020","journal-title":"Mol Ther Methods Clin Dev"},{"key":"2023031712550853000_2023.03.14.532624v1.25","unstructured":"J.-Y. 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