{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T18:50:11Z","timestamp":1768503011590,"version":"3.49.0"},"posted":{"date-parts":[[2023,3,18]]},"group-title":"Developmental Biology","reference-count":74,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,2,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  The acquisition of the post-mitotic state is crucial for the execution of many terminally differentiated cell behaviors during organismal development. However, the mechanisms that maintain the post-mitotic state in this context remain poorly understood. To gain insight into these mechanisms, we used the genetically and visually accessible model of\n                  <jats:italic>C. elegans<\/jats:italic>\n                  anchor cell (AC) invasion into the vulval epithelium. The AC is a terminally differentiated uterine cell that normally exits the cell cycle and enters a post-mitotic state, initiating contact between the uterus and vulva through a cell invasion event. Here, we set out to identify the set of negative cell cycle regulators that maintain the AC in this post-mitotic, invasive state. Our findings revealed a critical role for CKI-1 (p21\n                  <jats:sup>CIP1<\/jats:sup>\n                  \/p27\n                  <jats:sup>KIP1<\/jats:sup>\n                  ) in redundantly maintaining the post-mitotic state of the AC, as loss of CKI-1 in combination with other negative cell cycle regulators\u2014including CKI-2 (p21\n                  <jats:sup>CIP1<\/jats:sup>\n                  \/p27\n                  <jats:sup>KIP1<\/jats:sup>\n                  ), LIN-35 (pRb\/p107\/p130), FZR-1 (Cdh1\/Hct1), and LIN-23 (\u03b2-TrCP)\u2014resulted in proliferating ACs. Remarkably, time-lapse imaging revealed that these ACs retain their ability to invade. Upon examination of a node in the gene regulatory network controlling AC invasion, we determined that proliferating, invasive ACs do so by maintaining aspects of pro-invasive gene expression. We therefore report that the requirement for a post-mitotic state for invasive cell behavior can be bypassed following direct cell cycle perturbation.\n                <\/jats:p>","DOI":"10.1101\/2023.03.16.533034","type":"posted-content","created":{"date-parts":[[2023,3,19]],"date-time":"2023-03-19T03:51:36Z","timestamp":1679197896000},"source":"Crossref","is-referenced-by-count":0,"title":["Cell cycle perturbation uncouples mitotic progression and invasive behavior in a post-mitotic cell"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1178-7139","authenticated-orcid":false,"given":"Michael A. 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