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Here, we report mapping of connections between presynaptic and postsynaptic partners in a living vertebrate nervous system, that of the zebrafish, through the successful adaptation of the\n                  <jats:italic>trans<\/jats:italic>\n                  -Tango genetic approach, first developed for anterograde transsynaptic tracing in\n                  <jats:italic>Drosophila<\/jats:italic>\n                  . Neural connections were visualized between synaptic partners in the larval retina and brain and followed over development. Results were corroborated by functional experiments in which optogenetic activation of retinal ganglion cells elicited responses in neurons of the optic tectum, as measured by\n                  <jats:italic>trans<\/jats:italic>\n                  -Tango-dependent expression of a genetically encoded calcium indicator.\n                <\/jats:p>\n                <jats:p>\n                  Transsynaptic signaling through\n                  <jats:italic>trans<\/jats:italic>\n                  -Tango reveals predicted as well as previously undescribed synaptic connections in the zebrafish brain, providing a valuable\n                  <jats:italic>in vivo<\/jats:italic>\n                  tool to monitor and interrogate neural circuits over time.\n                <\/jats:p>","DOI":"10.1101\/2023.04.03.535421","type":"posted-content","created":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T23:05:16Z","timestamp":1680563116000},"source":"Crossref","is-referenced-by-count":4,"title":["Transsynaptic labeling and transcriptional control of zebrafish neural circuits"],"prefix":"10.64898","author":[{"given":"Cagney","family":"Coomer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daria","family":"Naumova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustafa","family":"Talay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bence","family":"Zolyomi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathaniel J.","family":"Snell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0739-6314","authenticated-orcid":false,"given":"Altar","family":"Sorka\u00e7","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Michale","family":"Chanchu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Roman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Drew","family":"Robson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilad","family":"Barnea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marnie E.","family":"Halpern","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023050809550714000_2023.04.03.535421v4.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2006.02.053"},{"key":"2023050809550714000_2023.04.03.535421v4.2","doi-asserted-by":"crossref","unstructured":"Luo, L. , Callaway, E.M. & Svoboda, K . 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