{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T10:43:46Z","timestamp":1780569826925,"version":"3.54.1"},"posted":{"date-parts":[[2023,4,6]]},"group-title":"Microbiology","reference-count":75,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,4,6]]},"abstract":"<jats:title>Summary paragraph<\/jats:title>\n                <jats:p>\n                  The host-microbiota relationship has evolved to shape mammalian processes, including immunity, metabolism, and development\n                  <jats:sup>1\u20133<\/jats:sup>\n                  . Host phenotypes change in direct response to microbial exposures by the individual. Here we show that the microbiota induces phenotypic change not only in the individual but also in their succeeding generations of progeny. We found that germ-free mice exhibit a robust sebum secretion defect and transcriptional changes in various organs, persisting across multiple generations despite microbial colonization and breeding with conventional mice. Host-microbe interactions could be involved in this process, since T cell-deficient mice, which display defective sebum secretion\n                  <jats:sup>4<\/jats:sup>\n                  , also transgenerationally transmit their phenotype to progeny. These phenotypes are inherited by progeny conceived during\n                  <jats:italic>in vitro<\/jats:italic>\n                  fertilization using germ-free sperm and eggs, demonstrating that epigenetic information in the gametes is required for phenotypic transmission. Accordingly, small non-coding RNAs that can regulate embryonic gene expression\n                  <jats:sup>5<\/jats:sup>\n                  were strikingly and similarly altered in gametes of germ-free and T cell-deficient mice. Thus, we have uncovered a novel mechanism whereby the microbiota and immune system induce phenotypic changes in successive generations of offspring. This epigenetic form of inheritance could be advantageous for host adaptation to environmental perturbation, where phenotypic diversity can be introduced more rapidly than by genetic mutation.\n                <\/jats:p>","DOI":"10.1101\/2023.04.06.535940","type":"posted-content","created":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T23:35:13Z","timestamp":1680824113000},"source":"Crossref","is-referenced-by-count":3,"title":["The microbiota and immune system non-genetically affect offspring phenotypes transgenerationally"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6859-7160","authenticated-orcid":false,"given":"Jordan C.","family":"Harris","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie A.","family":"Trigg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruktawit","family":"Goshu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichi","family":"Yokoyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenka","family":"Dohnalov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen K.","family":"White","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adele","family":"Harman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph A.","family":"Thaiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Grice","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin C.","family":"Conine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taku","family":"Kambayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2023041100250866000_2023.04.06.535940v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2015.10.016"},{"key":"2023041100250866000_2023.04.06.535940v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/nature25500"},{"key":"2023041100250866000_2023.04.06.535940v1.3","doi-asserted-by":"crossref","unstructured":"Leinwand, J. 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