{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:39:03Z","timestamp":1772156343630,"version":"3.50.1"},"posted":{"date-parts":[[2023,4,14]]},"group-title":"Pathology","reference-count":78,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,7,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Periodontitis, one of the most common non-communicable diseases, is characterized by chronic oral inflammation and uncontrolled tooth supporting alveolar bone resorption. Its underlying mechanism to initiate aberrant oral barrier immunity has yet to be delineated. Here, we report a unique fibroblast subpopulation\n                  <jats:underline>a<\/jats:underline>\n                  ctivated to\n                  <jats:underline>g<\/jats:underline>\n                  uide oral inflammation (AG fibroblasts) identified in a single-cell RNA sequencing gingival cell atlas constructed from the mouse periodontitis models. AG fibroblasts localized beneath the gingival epithelium and in the cervical periodontal ligament responded to the ligature placement and to the discrete application of Toll-like receptor stimulants to mouse maxillary tissue. The upregulated chemokines and ligands of AG fibroblasts linked to the putative receptors of neutrophils in the early stages of periodontitis. In the established chronic inflammation, neutrophils together with AG fibroblasts appeared to induce type 3 innate lymphoid cells (ILC3s) that were the primary source of interleukin-17 cytokines. The comparative analysis of\n                  <jats:italic>Rag2<\/jats:italic>\n                  -\/- and\n                  <jats:italic>Rag2\u03b3c<\/jats:italic>\n                  -\/-mice suggested that ILC3 contributed to the cervical alveolar bone resorption interfacing the gingival inflammation. We propose that AG fibroblasts function as a previously unrecognized surveillant to initiate gingival inflammation leading to periodontitis through the AG fibroblast-neutrophil-ILC3 axis.\n                <\/jats:p>","DOI":"10.1101\/2023.04.13.536751","type":"posted-content","created":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T03:05:19Z","timestamp":1681700719000},"source":"Crossref","is-referenced-by-count":1,"title":["Mouse gingival single-cell transcriptomic atlas: An activated fibroblast subpopulation guides oral barrier immunity in periodontitis"],"prefix":"10.64898","author":[{"given":"Takeru","family":"Kondo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annie","family":"Gleason","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroko","family":"Okawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akishige","family":"Hokugo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ichiro","family":"Nishimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023073023402665000_2023.04.13.536751v2.1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.174"},{"key":"2023073023402665000_2023.04.13.536751v2.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2017.04.008"},{"key":"2023073023402665000_2023.04.13.536751v2.3","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12729"},{"key":"2023073023402665000_2023.04.13.536751v2.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0089-x"},{"issue":"7","key":"2023073023402665000_2023.04.13.536751v2.5","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1111\/jcpe.13118","article-title":"Predictors 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