{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:39:06Z","timestamp":1772156346956,"version":"3.50.1"},"posted":{"date-parts":[[2023,4,20]]},"group-title":"Bioengineering","reference-count":54,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Each year, hundreds of millions of people are infected with arboviruses such as dengue, yellow fever, chikungunya, and Zika, which are all primarily spread by the notorious mosquito\n                  <jats:italic>Aedes aegypti<\/jats:italic>\n                  . Traditional control measures have proven insufficient, necessitating innovations. In response, here we generate a next generation CRISPR-based precision-guided sterile insect technique (pgSIT) for\n                  <jats:italic>Aedes aegypti<\/jats:italic>\n                  that disrupts genes essential for sex determination and fertility, producing predominantly sterile males that can be deployed at any life stage. Using mathematical models and empirical testing, we demonstrate that released pgSIT males can effectively compete with, suppress, and eliminate caged mosquito populations. This versatile species-specific platform has the potential for field deployment to effectively control wild populations of disease vectors.\n                <\/jats:p>","DOI":"10.1101\/2023.04.18.537404","type":"posted-content","created":{"date-parts":[[2023,4,20]],"date-time":"2023-04-20T10:02:24Z","timestamp":1681984944000},"source":"Crossref","is-referenced-by-count":3,"title":["Targeting Sex Determination to Suppress Mosquito Populations"],"prefix":"10.64898","author":[{"given":"Ming","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolay P.","family":"Kandul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruichen","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena D.","family":"Benetta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel J.","family":"Brogan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor","family":"Antoshechkin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9ctor M.","family":"S\u00e1nchez C.","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinpeng","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas A.","family":"DeBeaubien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YuMin M.","family":"Loh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew P.","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Craig","family":"Montell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John M.","family":"Marshall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6853-9884","authenticated-orcid":false,"given":"Omar S.","family":"Akbari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023111711550824000_2023.04.18.537404v2.1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.113.006742"},{"key":"2023111711550824000_2023.04.18.537404v2.2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001760"},{"key":"2023111711550824000_2023.04.18.537404v2.3","doi-asserted-by":"publisher","DOI":"10.1021\/bi016057v"},{"key":"2023111711550824000_2023.04.18.537404v2.4","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.1c01088"},{"key":"2023111711550824000_2023.04.18.537404v2.5","doi-asserted-by":"publisher","DOI":"10.3791\/61375"},{"key":"2023111711550824000_2023.04.18.537404v2.6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003864"},{"key":"2023111711550824000_2023.04.18.537404v2.7","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2015.34"},{"key":"2023111711550824000_2023.04.18.537404v2.8","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1093\/genetics\/139.4.1649","article-title":"A genetic analysis of intersex, a gene regulating sexual differentiation in Drosophila melanogaster females","volume":"139","year":"1995","journal-title":"Genetics"},{"key":"2023111711550824000_2023.04.18.537404v2.9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2105075118"},{"key":"2023111711550824000_2023.04.18.537404v2.10","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0471-x"},{"key":"2023111711550824000_2023.04.18.537404v2.11","doi-asserted-by":"publisher","DOI":"10.1101\/2023.01.27.525922"},{"key":"2023111711550824000_2023.04.18.537404v2.12","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA118.001067"},{"key":"2023111711550824000_2023.04.18.537404v2.13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1110717108"},{"key":"2023111711550824000_2023.04.18.537404v2.14","doi-asserted-by":"publisher","DOI":"10.1534\/g3.119.401006"},{"key":"2023111711550824000_2023.04.18.537404v2.15","doi-asserted-by":"crossref","first-page":"4661","DOI":"10.1242\/dev.129.20.4661","article-title":". intersex, a gene required for female sexual development in Drosophila, is expressed in both sexes and functions together with doublesex to regulate terminal differentiation","volume":"129","year":"2002","journal-title":"Development"},{"key":"2023111711550824000_2023.04.18.537404v2.16","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1318"},{"key":"2023111711550824000_2023.04.18.537404v2.17","doi-asserted-by":"publisher","DOI":"10.1126\/science.1078052"},{"key":"2023111711550824000_2023.04.18.537404v2.18","doi-asserted-by":"publisher","DOI":"10.1089\/crispr.2021.0077"},{"key":"2023111711550824000_2023.04.18.537404v2.19","doi-asserted-by":"publisher","DOI":"10.1534\/g3.119.400985"},{"key":"2023111711550824000_2023.04.18.537404v2.20","doi-asserted-by":"publisher","DOI":"10.1089\/genbio.2022.0019"},{"key":"2023111711550824000_2023.04.18.537404v2.21","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11617-8"},{"key":"2023111711550824000_2023.04.18.537404v2.22","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07964-7"},{"key":"2023111711550824000_2023.04.18.537404v2.23","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/48.4.459"},{"key":"2023111711550824000_2023.04.18.537404v2.24","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4245"},{"key":"2023111711550824000_2023.04.18.537404v2.25","doi-asserted-by":"publisher","DOI":"10.1038\/216383a0"},{"key":"2023111711550824000_2023.04.18.537404v2.26","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1711538114"},{"key":"2023111711550824000_2023.04.18.537404v2.27","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25421-w"},{"key":"2023111711550824000_2023.04.18.537404v2.28","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.51701"},{"key":"2023111711550824000_2023.04.18.537404v2.29","doi-asserted-by":"publisher","DOI":"10.1007\/s40726-020-00142-8"},{"key":"2023111711550824000_2023.04.18.537404v2.30","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2019.01072"},{"key":"2023111711550824000_2023.04.18.537404v2.31","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0692-z"},{"key":"2023111711550824000_2023.04.18.537404v2.32","doi-asserted-by":"publisher","DOI":"10.1111\/ele.13335"},{"key":"2023111711550824000_2023.04.18.537404v2.33","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004213"},{"key":"2023111711550824000_2023.04.18.537404v2.34","doi-asserted-by":"publisher","DOI":"10.1080\/20477724.2017.1453587"},{"key":"2023111711550824000_2023.04.18.537404v2.35","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-11-41"},{"key":"2023111711550824000_2023.04.18.537404v2.36","doi-asserted-by":"publisher","DOI":"10.3390\/toxics9080177"},{"key":"2023111711550824000_2023.04.18.537404v2.37","doi-asserted-by":"publisher","DOI":"10.1111\/2041-210X.13318"},{"key":"2023111711550824000_2023.04.18.537404v2.38","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.01819"},{"key":"2023111711550824000_2023.04.18.537404v2.39","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.ade8903"},{"key":"2023111711550824000_2023.04.18.537404v2.40","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abl4844"},{"key":"2023111711550824000_2023.04.18.537404v2.41","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.975786"},{"key":"2023111711550824000_2023.04.18.537404v2.42","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06388-7"},{"key":"2023111711550824000_2023.04.18.537404v2.43","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-04382-x"},{"key":"2023111711550824000_2023.04.18.537404v2.44","doi-asserted-by":"crossref","unstructured":"Trdan S. 2016. Insecticides Resistance. BoD \u2013 Books on Demand.","DOI":"10.5772\/60478"},{"key":"2023111711550824000_2023.04.18.537404v2.45","doi-asserted-by":"publisher","DOI":"10.3958\/059.046.0412"},{"key":"2023111711550824000_2023.04.18.537404v2.46","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24654-z"},{"key":"2023111711550824000_2023.04.18.537404v2.47","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25421-w"},{"key":"2023111711550824000_2023.04.18.537404v2.48","doi-asserted-by":"publisher","DOI":"10.1111\/2041-210X.13318"},{"key":"2023111711550824000_2023.04.18.537404v2.49","unstructured":"Molnar, C . Interpretable Machine Learning. (Lulu.com, 2020)."},{"key":"2023111711550824000_2023.04.18.537404v2.50","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/j.ress.2006.04.015","article-title":"A new uncertainty importance measure","volume":"92","year":"2007","journal-title":"Reliab. Eng. Syst. Saf."},{"key":"2023111711550824000_2023.04.18.537404v2.51","first-page":"3873","article-title":"Study of the sensitivity of coupled reaction systems to uncertainties in rate coefficients. I Theory","volume":"59","year":"2003","journal-title":"J. Chem. Phys"},{"key":"2023111711550824000_2023.04.18.537404v2.52","doi-asserted-by":"publisher","DOI":"10.2307\/1270993"},{"key":"2023111711550824000_2023.04.18.537404v2.53","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010933404324"},{"key":"2023111711550824000_2023.04.18.537404v2.54","unstructured":"Lundberg and Su-In Lee, S. A Unified Approach to Interpreting Model Predictions. https:\/\/proceedings.neurips.cc\/paper_files\/paper\/2017\/file\/8a20a8621978632d76c43dfd28b67767-Paper.pdf."}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2023.04.18.537404","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T04:34:05Z","timestamp":1768451645000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2023.04.18.537404"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,20]]},"references-count":54,"URL":"https:\/\/doi.org\/10.1101\/2023.04.18.537404","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.7554\/eLife.90199","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.90199.3","asserted-by":"object"}],"has-version":[{"id-type":"doi","id":"10.7554\/eLife.90199.1","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.90199.2","asserted-by":"object"}]},"subject":[],"published":{"date-parts":[[2023,4,20]]},"subtype":"preprint"}}