{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T19:06:04Z","timestamp":1768503964142,"version":"3.49.0"},"posted":{"date-parts":[[2023,5,2]]},"group-title":"Developmental Biology","reference-count":78,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,5,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Growth and specification of the mouse intestine occurs in utero and concludes after birth. While numerous studies have examined this developmental process in the small intestine, far less is known about the cellular and molecular cues required for colon development. In this study, we examine the morphological events leading to crypt formation, epithelial cell differentiation, areas of proliferation, and the emergence and expression of a stem and progenitor cell marker Lrig1. Through multicolor lineage tracing, we show Lrig1 expressing cells are present at birth and behave as stem cells to establish clonal crypts within three weeks after birth. In addition, we use an inducible knockout mouse to eliminate Lrig1 during colon development and show loss of Lrig1 restrains proliferation within a critical developmental time window, without impacting colonic epithelial cell differentiation. Our study illustrates the morphological changes that occur during crypt development and the importance of Lrig1 in the developing colon.<\/jats:p>","DOI":"10.1101\/2023.05.02.539114","type":"posted-content","created":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T20:35:31Z","timestamp":1683059731000},"source":"Crossref","is-referenced-by-count":0,"title":["The Role of Lrig1 in the Development of the Colonic Epithelium"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7450-3378","authenticated-orcid":false,"given":"Rachel E.","family":"Hopton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas J.","family":"Jahahn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8238-1406","authenticated-orcid":false,"given":"Anne E.","family":"Zemper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023050500300745000_2023.05.02.539114v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2020.103617"},{"key":"2023050500300745000_2023.05.02.539114v1.2","doi-asserted-by":"crossref","unstructured":"Kwon O , Han T-S , Son M-Y . Intestinal Morphogenesis in Development, Regeneration, and Disease: The Potential Utility of Intestinal Organoids for Studying Compartmentalization of the Crypt-Villus Structure [Online]. Front Cell Dev Biol 8, 2020. https:\/\/www.frontiersin.org\/articles\/10.3389\/fcell.2020.593969 [15 Jul. 2022].","DOI":"10.3389\/fcell.2020.593969"},{"key":"2023050500300745000_2023.05.02.539114v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(99)70193-9"},{"key":"2023050500300745000_2023.05.02.539114v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41390-019-0472-y"},{"key":"2023050500300745000_2023.05.02.539114v1.5","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22540"},{"key":"2023050500300745000_2023.05.02.539114v1.6","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2017.0217"},{"key":"2023050500300745000_2023.05.02.539114v1.7","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.12.042"},{"key":"2023050500300745000_2023.05.02.539114v1.8","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-020-00665-z"},{"key":"2023050500300745000_2023.05.02.539114v1.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.05.082"},{"key":"2023050500300745000_2023.05.02.539114v1.10","doi-asserted-by":"publisher","DOI":"10.1208\/s12248-017-0178-1"},{"key":"2023050500300745000_2023.05.02.539114v1.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2019.06.006"},{"key":"2023050500300745000_2023.05.02.539114v1.12","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-196901000-00004"},{"key":"2023050500300745000_2023.05.02.539114v1.13","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092110408"},{"key":"2023050500300745000_2023.05.02.539114v1.14","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092380309"},{"key":"2023050500300745000_2023.05.02.539114v1.15","doi-asserted-by":"publisher","DOI":"10.1038\/333463a0"},{"key":"2023050500300745000_2023.05.02.539114v1.16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027070"},{"key":"2023050500300745000_2023.05.02.539114v1.17","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2464"},{"key":"2023050500300745000_2023.05.02.539114v1.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.02.042"},{"key":"2023050500300745000_2023.05.02.539114v1.19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0078519"},{"key":"2023050500300745000_2023.05.02.539114v1.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2017.01.011"},{"key":"2023050500300745000_2023.05.02.539114v1.21","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2017.11.001"},{"key":"2023050500300745000_2023.05.02.539114v1.22","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.03.024"},{"key":"2023050500300745000_2023.05.02.539114v1.23","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201949224"},{"key":"2023050500300745000_2023.05.02.539114v1.24","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2184.2009.00642.x"},{"key":"2023050500300745000_2023.05.02.539114v1.25","doi-asserted-by":"publisher","DOI":"10.1038\/nature06196"},{"key":"2023050500300745000_2023.05.02.539114v1.26","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1294.001"},{"key":"2023050500300745000_2023.05.02.539114v1.27","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00073.2017"},{"key":"2023050500300745000_2023.05.02.539114v1.28","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-09927-2"},{"key":"2023050500300745000_2023.05.02.539114v1.29","doi-asserted-by":"publisher","DOI":"10.1038\/ng.165"},{"key":"2023050500300745000_2023.05.02.539114v1.30","doi-asserted-by":"publisher","DOI":"10.1126\/science.1213214"},{"key":"2023050500300745000_2023.05.02.539114v1.31","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038965"},{"key":"2023050500300745000_2023.05.02.539114v1.32","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1013004108"},{"key":"2023050500300745000_2023.05.02.539114v1.33","doi-asserted-by":"publisher","DOI":"10.1038\/333461a0"},{"key":"2023050500300745000_2023.05.02.539114v1.34","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.09.016"},{"key":"2023050500300745000_2023.05.02.539114v1.35","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.12.036"},{"key":"2023050500300745000_2023.05.02.539114v1.36","doi-asserted-by":"publisher","DOI":"10.1002\/embr.201337799"},{"key":"2023050500300745000_2023.05.02.539114v1.37","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-29230-y"},{"key":"2023050500300745000_2023.05.02.539114v1.38","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2013.04.001"},{"key":"2023050500300745000_2023.05.02.539114v1.39","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108257"},{"key":"2023050500300745000_2023.05.02.539114v1.40","doi-asserted-by":"publisher","DOI":"10.1186\/s13064-020-00139-5"},{"key":"2023050500300745000_2023.05.02.539114v1.41","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"2023050500300745000_2023.05.02.539114v1.42","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2012.150"},{"key":"2023050500300745000_2023.05.02.539114v1.43","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2184.1992.tb01435.x"},{"key":"2023050500300745000_2023.05.02.539114v1.44","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.04.018"},{"key":"2023050500300745000_2023.05.02.539114v1.45","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2014.09.002"},{"key":"2023050500300745000_2023.05.02.539114v1.46","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2009.04.014"},{"key":"2023050500300745000_2023.05.02.539114v1.47","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601886103"},{"key":"2023050500300745000_2023.05.02.539114v1.48","doi-asserted-by":"publisher","DOI":"10.1038\/nature10163"},{"key":"2023050500300745000_2023.05.02.539114v1.49","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2013.138"},{"key":"2023050500300745000_2023.05.02.539114v1.50","doi-asserted-by":"publisher","DOI":"10.1530\/ERC-14-0179"},{"key":"2023050500300745000_2023.05.02.539114v1.51","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.807487"},{"key":"2023050500300745000_2023.05.02.539114v1.52","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1999.276.3.G666"},{"key":"2023050500300745000_2023.05.02.539114v1.53","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(86)90202-2"},{"key":"2023050500300745000_2023.05.02.539114v1.54","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/823897"},{"key":"2023050500300745000_2023.05.02.539114v1.55","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.06.072"},{"key":"2023050500300745000_2023.05.02.539114v1.56","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.16.3457"},{"key":"2023050500300745000_2023.05.02.539114v1.57","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01576"},{"key":"2023050500300745000_2023.05.02.539114v1.58","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1205669109"},{"key":"2023050500300745000_2023.05.02.539114v1.59","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2020.02.003"},{"key":"2023050500300745000_2023.05.02.539114v1.60","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.22.10335"},{"key":"2023050500300745000_2023.05.02.539114v1.61","doi-asserted-by":"publisher","DOI":"10.1038\/nature09637"},{"key":"2023050500300745000_2023.05.02.539114v1.62","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1002491"},{"key":"2023050500300745000_2023.05.02.539114v1.63","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1607327113"},{"key":"2023050500300745000_2023.05.02.539114v1.64","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0229"},{"key":"2023050500300745000_2023.05.02.539114v1.65","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2020.05.009"},{"key":"2023050500300745000_2023.05.02.539114v1.66","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2012.149"},{"key":"2023050500300745000_2023.05.02.539114v1.67","doi-asserted-by":"publisher","DOI":"10.1038\/nature22313"},{"key":"2023050500300745000_2023.05.02.539114v1.68","doi-asserted-by":"publisher","DOI":"10.1038\/ng1430"},{"key":"2023050500300745000_2023.05.02.539114v1.69","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13824"},{"key":"2023050500300745000_2023.05.02.539114v1.70","doi-asserted-by":"publisher","DOI":"10.1038\/nature03659"},{"key":"2023050500300745000_2023.05.02.539114v1.71","doi-asserted-by":"publisher","DOI":"10.1038\/nature03589"},{"key":"2023050500300745000_2023.05.02.539114v1.72","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2010.39"},{"key":"2023050500300745000_2023.05.02.539114v1.73","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1295405"},{"key":"2023050500300745000_2023.05.02.539114v1.74","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2015.03.012"},{"key":"2023050500300745000_2023.05.02.539114v1.75","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.17.7868-7878.2005"},{"key":"2023050500300745000_2023.05.02.539114v1.76","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20741"},{"key":"2023050500300745000_2023.05.02.539114v1.77","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.05.033"},{"key":"2023050500300745000_2023.05.02.539114v1.78","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.180120"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2023.05.02.539114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T04:36:52Z","timestamp":1768451812000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2023.05.02.539114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,2]]},"references-count":78,"URL":"https:\/\/doi.org\/10.1101\/2023.05.02.539114","relation":{},"subject":[],"published":{"date-parts":[[2023,5,2]]},"subtype":"preprint"}}