{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:46:39Z","timestamp":1790739999083,"version":"4.1.0"},"posted":{"date-parts":[[2023,5,8]]},"group-title":"Microbiology","reference-count":45,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,5,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Fluid flow is thought to prevent bacterial adhesion, but some bacteria use adhesins with catch bond properties to enhance adhesion under high shear forces. However, many studies on bacterial adhesion either neglect the influence of shear force or use shear forces that are not typically found in natural systems. In this study, we use microfluidics and single-cell imaging to examine how the human pathogen\n                  <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                  interacts with surfaces when exposed to shear forces typically found in the human body (0.1 pN to 10 pN). Through cell tracking, we demonstrate that the angle between the cell and the surface predicts if a cell will depart the surface. We discover that at lower shear forces, type IV pilus retraction tilts cells away from the surface, promoting surface departure. Conversely, we show that higher shear forces counterintuitively enhance adhesion by counteracting type IV pilus retraction-dependent cell tilting. Thus, our results reveal that\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  exhibits behavior reminiscent of a catch bond, without having a specific adhesin that is enhanced by force. Instead,\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  couples type IV pilus dynamics and cell geometry to tune adhesion to its mechanical environment, which likely provides a benefit in dynamic host environments.\n                <\/jats:p>","DOI":"10.1101\/2023.05.08.539440","type":"posted-content","created":{"date-parts":[[2023,5,9]],"date-time":"2023-05-09T00:50:11Z","timestamp":1683593411000},"source":"Crossref","is-referenced-by-count":3,"title":["Shear force enhances adhesion of\n                  <i>Pseudomonas aeruginosa<\/i>\n                  by counteracting pilus-driven surface departure"],"prefix":"10.64898","author":[{"given":"Jessica-Jae S.","family":"Palalay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmet N.","family":"Simsek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7769-8043","authenticated-orcid":false,"given":"Benedikt","family":"Sabass","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph E.","family":"Sanfilippo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2023051020251264000_2023.05.08.539440v1.1","doi-asserted-by":"crossref","first-page":"2851","DOI":"10.1038\/s41467-020-16620-y","article-title":"The effect of flow on swimming bacteria controls the initial colonization of curved surfaces","volume":"11","year":"2020","journal-title":"Nat. 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