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Here, we engineered\n                  <jats:italic>E. coli<\/jats:italic>\n                  to titrate H\n                  <jats:sub>2<\/jats:sub>\n                  S controllably across the physiological range in a gut microphysiological system (chip) supportive of the co-culture of microbes and host cells. The chip was designed to maintain H\n                  <jats:sub>2<\/jats:sub>\n                  S gas tension and enable visualization of co-culture in real-time with confocal microscopy. Engineered strains colonized the chip and were metabolically active for two days, during which they produced H\n                  <jats:sub>2<\/jats:sub>\n                  S across a sixteen-fold range and induced changes in host gene expression and metabolism in an H\n                  <jats:sub>2<\/jats:sub>\n                  S concentration-dependent manner. These results validate a novel platform for studying the mechanisms underlying microbe-host interactions, by enabling experiments that are infeasible with current animal and\n                  <jats:italic>in vitro<\/jats:italic>\n                  models.\n                <\/jats:p>","DOI":"10.1101\/2023.05.16.538950","type":"posted-content","created":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T00:05:13Z","timestamp":1684281913000},"source":"Crossref","is-referenced-by-count":1,"title":["Engineered bacteria titrate hydrogen sulfide and induce concentration-dependent effects on host in a gut microphysiological system"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2531-8333","authenticated-orcid":false,"given":"Justin A.","family":"Hayes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna W.","family":"Lunger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aayushi S.","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew T.","family":"Fernez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0433-9290","authenticated-orcid":false,"given":"Abigail N.","family":"Koppes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3376-6358","authenticated-orcid":false,"given":"Ryan","family":"Koppes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin M.","family":"Woolston","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023051820501225000_2023.05.16.538950v1.1","first-page":"69","article-title":"The gut microbiome in health and in disease","volume":"31","year":"2015","journal-title":"Cell"},{"key":"2023051820501225000_2023.05.16.538950v1.2","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1007\/s10354-017-0592-0","article-title":"Intestinal microbiome-gut-brain axis and irritable bowel syndrome","volume":"168","year":"2018","journal-title":"Wiener Medizinische Wochenschrift"},{"key":"2023051820501225000_2023.05.16.538950v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-019-0258-z"},{"key":"2023051820501225000_2023.05.16.538950v1.4","doi-asserted-by":"crossref","first-page":"807648","DOI":"10.3389\/fimmu.2021.807648","article-title":"Potential Role of the Gut Microbiome In Colorectal Cancer Progression","volume":"12","year":"2022","journal-title":"Front. 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