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We developed a virtual reality place preference paradigm in which head-fixed mice can voluntarily seek a starvation-like state induced by optogenetic stimulation of hypothalamic agouti-related peptide (AgRP) neurons. Prior to stress induction, male but not female mice showed mild aversion to AgRP stimulation. Strikingly, following chronic stress, a subset of females developed a strong preference for AgRP stimulation that was predicted by high baseline anxiety. Such stress-induced changes in preference were reflected in changes in facial expressions during AgRP stimulation. Our study suggests that stress may cause females predisposed to anxiety to seek a starvation state, and provides a powerful experimental framework for investigating the underlying neural mechanisms.<\/jats:p>","DOI":"10.1101\/2023.05.16.541013","type":"posted-content","created":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T00:05:25Z","timestamp":1684368325000},"source":"Crossref","is-referenced-by-count":1,"title":["Chronic stress triggers seeking of a starvation-like state in anxiety-prone female mice"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8175-0564","authenticated-orcid":false,"given":"Hakan","family":"Kucukdereli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8070-0378","authenticated-orcid":false,"given":"Oren","family":"Amsalem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3522-6109","authenticated-orcid":false,"given":"Trent","family":"Pottala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Lim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leilani","family":"Potgieter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda","family":"Hasbrouck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6991-2898","authenticated-orcid":false,"given":"Andrew","family":"Lutas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark L.","family":"Andermann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2023052002100950000_2023.05.16.541013v1.1","doi-asserted-by":"publisher","DOI":"10.1007\/s11920-012-0282-y"},{"key":"2023052002100950000_2023.05.16.541013v1.2","doi-asserted-by":"publisher","DOI":"10.1001\/archgenpsychiatry.2011.74"},{"key":"2023052002100950000_2023.05.16.541013v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2007.11.037"},{"key":"2023052002100950000_2023.05.16.541013v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2013.01.003"},{"key":"2023052002100950000_2023.05.16.541013v1.5","doi-asserted-by":"publisher","DOI":"10.1002\/eat.20244"},{"key":"2023052002100950000_2023.05.16.541013v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2006.03.040"},{"key":"2023052002100950000_2023.05.16.541013v1.7","doi-asserted-by":"publisher","DOI":"10.1176\/appi.ajp.161.12.2215"},{"key":"2023052002100950000_2023.05.16.541013v1.8","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2682"},{"key":"2023052002100950000_2023.05.16.541013v1.9","doi-asserted-by":"publisher","DOI":"10.2147\/NDT.S108912"},{"key":"2023052002100950000_2023.05.16.541013v1.10","doi-asserted-by":"publisher","DOI":"10.1002\/EAT.23265"},{"key":"2023052002100950000_2023.05.16.541013v1.11","doi-asserted-by":"publisher","DOI":"10.1002\/eat.22345"},{"key":"2023052002100950000_2023.05.16.541013v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2021.06.020"},{"key":"2023052002100950000_2023.05.16.541013v1.13","doi-asserted-by":"publisher","DOI":"10.1037\/h0025205"},{"key":"2023052002100950000_2023.05.16.541013v1.14","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2019.00069"},{"key":"2023052002100950000_2023.05.16.541013v1.15","doi-asserted-by":"publisher","DOI":"10.1038\/tp.2016.35"},{"key":"2023052002100950000_2023.05.16.541013v1.16","doi-asserted-by":"publisher","DOI":"10.1126\/science.1129663"},{"key":"2023052002100950000_2023.05.16.541013v1.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2011.07.044"},{"key":"2023052002100950000_2023.05.16.541013v1.18","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mp.4001281"},{"key":"2023052002100950000_2023.05.16.541013v1.19","doi-asserted-by":"publisher","DOI":"10.1007\/s11920-022-01319-2"},{"key":"2023052002100950000_2023.05.16.541013v1.20","doi-asserted-by":"publisher","DOI":"10.3389\/fnhum.2020.596381"},{"key":"2023052002100950000_2023.05.16.541013v1.21","doi-asserted-by":"publisher","DOI":"10.1097\/HRP.0000000000000111"},{"key":"2023052002100950000_2023.05.16.541013v1.22","unstructured":"Hull, C.L . 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